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Mass-spectrometry-based microbial metabolomics: recent developments and applications

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Abstract

Metabolomics is an omics technique aiming at qualitatively and quantitatively describing a metabolome by various analytical platforms. It is an indispensable component of modern systems biology. Microbial metabolomics can be roughly classified as metabolic footprint analysis and metabolic fingerprint analysis depending on the analyte origins. Both of them have been beneficial to microbiological research for different reasons. Mass spectrometry and nuclear magnetic resonance spectroscopy techniques are popular analytical strategies prevailing in the metabolomics field. In this review, chromatography–mass-spectrometry-based microbial metabolomic analysis steps are summarized, including sample collection, metabolite extraction, instrument analysis, and data analysis. Moreover, their applications in some representative fields are discussed as examples. The aim of this review is to present briefly recent technical advances in mass-spectrometry-based analysis, and to highlight the value of modern applications of microbial metabolomics.

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Acknowledgments

The study was supported by the National Basic Research Program (2007CB707800) of the State Ministry of Science and Technology of China, the Knowledge Innovation Program of the Chinese Academy of Sciences, the Foundation (no. 21175132), the Creative Research Group Project (no. 21321064), and the Surface Project (81372695) from the National Natural Science Foundation of China.

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  1. Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China

    Peng Gao & Guowang Xu

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Correspondence to Guowang Xu.

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Gao, P., Xu, G. Mass-spectrometry-based microbial metabolomics: recent developments and applications. Anal Bioanal Chem 407, 669–680 (2015). https://doi.org/10.1007/s00216-014-8127-7

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