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Asymmetrical flow field-flow fractionation with multi-angle light scattering and quasi-elastic light scattering for characterization of polymersomes: comparison with classical techniques

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Abstract

Polymersomes formed from amphiphilic block copolymers, such as poly(ethyleneoxide-b-ε-caprolactone) (PEO-b-PCL) or poly(ethyleneoxide-b-methylmethacrylate), were characterized by asymmetrical flow field-flow fractionation coupled with quasi-elastic light scattering (QELS), multi-angle light scattering (MALS), and refractive index detection, leading to the determination of their size, shape, and molecular weight. The method was cross-examined with more classical ones, like batch dynamic and static light scattering, electron microscopy, and atomic force microscopy. The results show good complementarities between all the techniques; asymmetrical flow field-flow fractionation being the most pertinent one when the sample exhibits several different types of population.

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Acknowledgments

The authors wish to thank the French ANR (ANR COPOPDT), PRES Toulouse and Midi-Pyrénées Region for funding and PhD grant for U. Till. EU (FEDER-35477: Nano-objets pour la biotechnologie) is greatly acknowledged for financial support (AFM instrument). LLB is thanked for beam time access and Annie Brûlet for fruitful discussion.

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Authors and Affiliations

  1. Université de Toulouse, UPS/CNRS, IMRCP, 118 route de Narbonne, 31062, Toulouse Cedex 9, France

    Ugo Till, Glenn Hamon, Jean-Daniel Marty & Anne-Françoise Mingotaud

  2. Université de Toulouse, Institut National Polytechnique de Toulouse–Ecole d’Ingénieurs de Purpan, Département Sciences Agronomiques et Agroalimentaires, UPSP/DGER 115, 75 voie du TOEC, BP 57611, 31076, Toulouse Cedex 03, France

    Ugo Till, Mireille Gaucher-Delmas & Frédéric Violleau

  3. Technopolym, Institut de Chimie de Toulouse, 118 route de Narbonne, 31062, Toulouse Cedex 9, France

    Pascale Saint-Aguet

  4. LUNAM Université, Université du Maine, IMMM UMR CNRS 6283, Département PCI, Avenue Olivier Messiaen, 72085, Le Mans Cedex 09, France

    Christophe Chassenieux

  5. Centre de Microscopie Electronique Appliquée à la Biologie, Faculté de Médecine Toulouse Rangueil, Université de Toulouse, 133, route de Narbonne, 31062, Toulouse cedex 4, France

    Bruno Payré & Dominique Goudounèche

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  1. Ugo Till
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  2. Mireille Gaucher-Delmas
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  8. Dominique Goudounèche
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  9. Anne-Françoise Mingotaud
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  10. Frédéric Violleau
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Corresponding authors

Correspondence to Anne-Françoise Mingotaud or Frédéric Violleau.

Additional information

Published in the topical collection Field- and Flow-based Separations with guest editors Gaetane Lespes, Catia Contado, and Bruce Gale.

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Till, U., Gaucher-Delmas, M., Saint-Aguet, P. et al. Asymmetrical flow field-flow fractionation with multi-angle light scattering and quasi-elastic light scattering for characterization of polymersomes: comparison with classical techniques. Anal Bioanal Chem 406, 7841–7853 (2014). https://doi.org/10.1007/s00216-014-7891-8

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  • DOI: https://doi.org/10.1007/s00216-014-7891-8

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