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Sensitive detection of trace hemoglobin using fluorescence method based on functionalized quantum dots

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Abstract

The fluorescence quenching of quantum dots by hemoglobin has been demonstrated to depend on surface functionalization, and this property has been utilized to construct a novel fluorescent method for rapid, sensitive, and selective detection of trace hemoglobin in urine at microgram level. This method shows low interference and high selectivity for hemoglobin with a limit of detection of 4.3 μg L−1 in water and 66.1 μg L−1 in urine, which are lower than those of currently used methods in labs and clinics. Spike and recovery tests in raw, acidified, and alkalized urine samples exhibit good recovery rates for the spiked concentrations close to the limit of detection.

Fluorescence spectra and photographs of MPA-QD solution before and after the addition of Hb taken under 365-nm irradiation.

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Acknowledgments

This work was supported by the Natural Science Foundation of China (nos. 21077108, 21173229) and the Innovation Project of the Chinese Academy of Sciences (KJCX2-YW-H29).

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Authors and Affiliations

  1. Department of Chemistry, University of Science and Technology of China, Hefei, Anhui, 230026, China

    Houjuan Zhu & Suhua Wang

  2. Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, Anhui, 230031, China

    Houjuan Zhu & Suhua Wang

Authors
  1. Houjuan Zhu
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  2. Suhua Wang
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Correspondence to Suhua Wang.

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Zhu, H., Wang, S. Sensitive detection of trace hemoglobin using fluorescence method based on functionalized quantum dots. Anal Bioanal Chem 405, 4989–4991 (2013). https://doi.org/10.1007/s00216-013-6832-2

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  • DOI: https://doi.org/10.1007/s00216-013-6832-2

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