Abstract
The Consultative committee for amount of substance-metrology in chemistry (CCQM)-K80 Key Comparison directly assessed the equivalence of many of the world’s higher-order value-assigned materials (HOVAMs) for creatinine in human serum. This 2009 international study compared the certified values and uncertainties of the materials using measurements made under repeatability conditions. The study evaluated 17 materials submitted by 6 national metrology institutes (NMIs). The creatinine quantity in these materials ranged from 3 mg/kg to 57 mg/kg (about 0.3 mg/dL to 6 mg/dL or 30 nmol/L to 500 nmol/L). All materials were stored and prepared according the specifications provided by the participating NMIs. Samples were processed and analyzed under repeatability conditions by one analyst using isotope-dilution liquid chromatography–mass spectrometry in two measurement campaigns. The certified values and repeatability measurements were compared using uncertainty-weighted generalized distance regression. The instrumental repeatability relative standard deviation was 1.2%. The measurement design required assessment of within-unit and between-campaign variability in addition to measurement repeatability. At a 95% level of confidence, the certified values for all 17 materials agreed to within their assigned uncertainties. CCQM-K80 demonstrated the metrological equivalence of the currently available HOVAMs for creatinine in human serum and of the creatinine measurement services provided by the participating NMIs.
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Notes
Certain commercial materials are identified in this report to specify the experimental procedure as completely as possible. In no case does such identification imply a recommendation or endorsement by the National Institute of Standards and Technology, nor does it imply that the materials are necessarily the best available for the purpose.
Abbreviations
- CCQM:
-
Consultative Committee for Amount of Substance–Metrology in Chemistry
- CENAM:
-
Centro Nacional de Metrología (Mexico)
- CRM:
-
Certified reference material
- GDR:
-
Generalized distance regression
- GUM:
-
Guide to the expression of uncertainty in measurement
- HOVAM:
-
Higher-order value-assigned material
- KC:
-
Key comparison
- KRISS:
-
Korea Research Institute of Standards and Science (Korea)
- LC:
-
Liquid chromatography
- LC-IDMS:
-
Liquid chromatography-isotope dilution mass spectrometry
- LGC:
-
Limited (UK)
- LOO:
-
Leave one out cross-validation
- NIM:
-
National Institute of Metrology (China)
- NIST:
-
National Institute of Standards and Technology (USA)
- NKDEP:
-
National Kidney Disease Education Program
- NMI:
-
National metrology institute
- OAWG:
-
Organic analysis working group
- PBMC:
-
Parametric bootstrap Monte Carlo
- PT:
-
Proficiency test
- PTB:
-
Physikalisch–Technische Bundesanstalt (Germany)
- SIM:
-
Selected ion monitoring
References
Stokes P, O'Connor G (2003) Development of a liquid chromatography-mass spectrometry method for the high-accuracy determination of creatinine in serum. J Chrom B 794:125–136
Van Nevel L, Ornemark U, Smeyers P, Harper C, Taylor PDP (2002) IMEP-17 report to participants. Part 1 International comparability
Myers GL, Miller WG, Coresh J, Fleming J, Greenberg N, Greene T et al (2006) Recommendations for improving serum creatinine measurement: a report from the laboratory working group of the National Kidney Disease Education Program. Clin Chem 52:5–18
Welch MJ, Phinney CP, Parris RM, May WE, Heo GS, Henrion A et al (2003) CCQM-K12: the determination of creatinine in serum. Metrologia 40:08005
JCGM 100:2008 (2008) Guide to the expression of uncertainty in measurement (GUM). Sèvres, France: BIPM http://www.bipm.org/utils/common/documents/jcgm/JCGM_100_2008_E.pdf
JCGM 101:2008 (2008) Evaluation of measurement data–Supplement 1 to the GUM—Propagation of distributions using a Monte Carlo method. Sèvres, France: BIPM http://www.bipm.org/utils/common/documents/jcgm/JCGM_101_2008_E.pdf
Lunn DJ, Thomas A, Best N, Spiegelhalter D (2000) WinBUGS—a Bayesian modelling framework: concepts, structure, and extensibility. Stat Comput 10:325–337
ISO 6143:2001 (2001) Gas analysis–comparison methods for determining and checking the composition of calibration gas mixtures. International Organization for Standardization, Geneva
van der Veen AMH, Brinkmann FNC, Arnautovic M, Besley L, Heine HJ, Estban TL et al (2007) International comparison CCQM-P41 Greenhouse gases. 2. Direct comparison of primary standard gas mixtures. Metrologia 44:08003
Wielgosz RI, Esler M, Viallon J, Moussay P, Oh SH, Kim BM et al (2008) Final report on CCQM-P73: international comparison of nitrogen monoxide in nitrogen gas standards (30–70) μmol/mol. Metrologia 45:08002
van der Veen AMH, Chander H, Ziel PR, Wessel RM, de Leer EWB, Smeulders D et al (2010) International comparison CCQM-K54: primary standard gas mixtures of hexane in methane. Metrologia 47:08019
Lee J, Lee JB, Moon DM, Kim JS, van der Veen AMH, Besley L et al (2010) Final report on international key comparison CCQM-K53: oxygen in nitrogen. Metrologia 47:08005
Duewer DL, Lippa K, Long S, Murphy K, Sharpless K, Sniegoski L et al (2009) Demonstrating the comparability of certified reference materials. Anal Bioanal Chem 395:155–169
Duewer DL, Kowalski BR, Fasching JL (1976) Improving reliability of factor-analysis of chemical data by utilizing measured analytical uncertainty. Anal Chem 48:2002–2010
Picard RR, Cook RD (1984) Cross-validation of regression-models. J Am Stat Assoc 79:575–583
Finney H, Newman DJ, Thakkar H, Fell JME, Price CP (2000) Reference ranges for plasma cystatin C and creatinine measurements in premature infants, neonates, and older children. Arch Dis Child 82:71–75
Ceriotti F, Boyd JC, Klein G, Henny J, Queralto J, Kairisto V et al (2008) Reference intervals for serum creatinine concentrations: assessment of available data for global application. Clin Chem 54:559–566
Acknowledgments
The following individuals are acknowledged for their contributions to CCQM-K80: William F. Guthrie, Reenie Parris, Karen W. Phinney, Antonio Possolo, Katherine E. Sharpless, Jolene D. Splett, Melina Pérez Urquiza, Sang-Ryoul Park, Gavin O’Connor, Xinhua Dai, Ruediger Ohlendorf, and André Henrion.
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Camara, J.E., Lippa, K.A., Duewer, D.L. et al. An international assessment of the metrological equivalence of higher-order measurement services for creatinine in serum. Anal Bioanal Chem 403, 527–535 (2012). https://doi.org/10.1007/s00216-012-5869-y
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DOI: https://doi.org/10.1007/s00216-012-5869-y