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Orientation of molecular groups of fibers in nonoriented samples determined by polarized ATR-FTIR spectroscopy

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Abstract

A method based on polarized attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy is proposed for determining the infrared dichroic absorption ratio of a single fiber from a sample deposited flat on a germanium crystal without the requirement of fiber orientation. The method shows its efficiency on cellulose fibers of paper and has been applied to protein fibers (type I collagen and β-amyloid) and polysaccharide fibers (cellulose and starch). The method gives access to the dichroic ratio of strong absorptions bands, which is not easily accessible with conventional absorption techniques. Then, the orientation of the molecular groups of organic fibers can be easily determined by polarized ATR-FTIR spectroscopy. By extension, this method will be useful to determine the molecular orientation of fibers in structured complex samples, such as biological tissues and plants. Spatially resolved information on the organization of the fiber network will be easily extracted by utilizing a focal plane array detector for imaging measurements.

Type I collagen and cellulose fibers orientation determined by polarized ATR-FTIR spectroscopy. Non-oriented fiber samples deposited on ATR crystal can be used to determine molecular groups orientation in fibers with p- an s-polarization spectra. The method can be extended to complex samples containing different fiber compounds.

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Authors and Affiliations

  1. Laboratoire de Chimie et Biologie des Membranes et Nano-objets, Université de Bordeaux, CNRS UMR 5084, 146 rue Léo Saignat, 33076, Bordeaux, France

    Karima Belbachir, Sophie Lecomte, Ha-Phuong Ta, Cyril Petibois & Bernard Desbat

  2. Geoffroy Saint-Hilaire, 33600, Pessac-Cedex, France

    Cyril Petibois

Authors
  1. Karima Belbachir
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  2. Sophie Lecomte
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  3. Ha-Phuong Ta
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  4. Cyril Petibois
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  5. Bernard Desbat
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Correspondence to Cyril Petibois.

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Belbachir, K., Lecomte, S., Ta, HP. et al. Orientation of molecular groups of fibers in nonoriented samples determined by polarized ATR-FTIR spectroscopy. Anal Bioanal Chem 401, 3263–3268 (2011). https://doi.org/10.1007/s00216-011-5418-0

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  • DOI: https://doi.org/10.1007/s00216-011-5418-0

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