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Chemiluminescence aptasensor for cocaine based on double-functionalized gold nanoprobes and functionalized magnetic microbeads

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Abstract

A novel chemiluminescence (CL) aptasensor for highly sensitive detection of small-molecule targets using cocaine as a model analyte was developed in the present study. For the proposed aptasensor, the aptamers were immobilized on the surface of gold nanoparticles (AuNPs) functionalized magnetic microbeads (MB-AuNPs) and then hybridized with the signal DNA on the double-functional gold nanoprobes (DF-AuNPs) modified with horseradish peroxidase (HRP). When cocaine was introduced, a competition for the aptamer between cocaine and the signal DNA occurred and the gold nanoprobes were forced to dissociate from the MB-AuNPs surface based on the structure switching of the aptamer. The CL signals of Luminol-H2O2-HRP-PIP system were proportional to the concentration of cocaine. A linear range was obtained when the concentrations of cocaine were from 1 × 10−9 to 1 × 10−8 M with the detection limit of 0.48 nM (3σ), much lower than those achieved by other methods. This new system can be easily extended to a variety of small-molecules, protein, and tumor cell analysis.

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Acknowledgment

This work was supported by the Education Administration Foundation of Shandong Province (No. J09LB04).

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Authors and Affiliations

  1. College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 210009, China

    Ying Li

  2. Shandong Provincial Key Laboratory Of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China

    Ying Li, Xiaoting Ji & Bangwei Liu

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  1. Ying Li
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  2. Xiaoting Ji
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Correspondence to Ying Li.

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Li, Y., Ji, X. & Liu, B. Chemiluminescence aptasensor for cocaine based on double-functionalized gold nanoprobes and functionalized magnetic microbeads. Anal Bioanal Chem 401, 213–219 (2011). https://doi.org/10.1007/s00216-011-5064-6

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  • DOI: https://doi.org/10.1007/s00216-011-5064-6

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