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Detection of collagens in brain tumors based on FTIR imaging and chemometrics

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Abstract

Fourier transform infrared (FTIR) imaging has been used as a molecular histopathology tool on brain tissue sections after intracranial implantation and development of glioma tumors. Healthy brain tissue (contralateral lobe) as well as solid and diffuse tumor tissues were compared for their collagen contents. IR spectra were extracted from IR images for determining the secondary structure of protein contents and compared to pure product spectra of collagens (types I, III, IV, V, and VI). Multivariate statistical analyses of variance and correspondence factorial analysis were performed to differentiate healthy and tumor brain tissues as well as their classification according to their secondary structure profiles. Secondary structure profiles revealed that no collagen was present in healthy tissues; they are also significantly different from solid and diffuse tumors (p < 0.05). Solid and diffuse tumors could be discriminated with respect to the secondary structure profile of fibrillar and non-fibrillar collagens, respectively. We can thus propose to develop FTIR imaging for histopathology examination of tumors on the basis of collagen contents.

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Acknowledgments

The authors are indebted to the ‘Italian–French University” (Vinci program 2009), the “Ligue Contre le Cancer,” the “Association Française contre les Myopathies,” and the “Agence Nationale pour la Recherche–ANR” for their financial supports.

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Authors and Affiliations

  1. Université de Bordeaux, CNRS UMR 5248, B8 Avenue des Facultés, 33402, Talence Cedex, France

    Razia Noreen, Seydou Yao & Cyril Petibois

  2. Institute of Physics, Academia Sinica, Nankang, Taipei, 115, Taiwan, Republic of China

    Chia-Chi Chien & Yeukuang Hwu

  3. Université de Bordeaux, Inserm U920, B2 Avenue des Facultés, 33402, Talence Cedex, France

    Maylis Delugin, Raphael Pineau & Michel Moenner

Authors
  1. Razia Noreen
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  2. Chia-Chi Chien
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  3. Maylis Delugin
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  4. Seydou Yao
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  5. Raphael Pineau
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  6. Yeukuang Hwu
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  7. Michel Moenner
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  8. Cyril Petibois
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Correspondence to Cyril Petibois.

Additional information

Published in the special issue Imaging Techniques with Synchrotron Radiation with Guest Editor Cyril Petibois.

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Noreen, R., Chien, CC., Delugin, M. et al. Detection of collagens in brain tumors based on FTIR imaging and chemometrics. Anal Bioanal Chem 401, 845–852 (2011). https://doi.org/10.1007/s00216-011-4899-1

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  • DOI: https://doi.org/10.1007/s00216-011-4899-1

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