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Applicability of a yeast bioassay in the detection of steroid esters in hair

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Abstract

The aim of the present study was to demonstrate the applicability of a yeast androgen and estrogen bioassay in the detection of steroid esters in hair samples of animals treated with a hormone ester cocktail. The outcome of the activity screenings was critically compared with the results previously obtained with LC-MS/MS analysis. Hair samples of one pour-on treated animal, 10 ml DMSO containing 25 mg estradiol benzoate (EB), 60 mg testosterone decanoate (TD) and 60 mg testosterone cypionate (TC), were selected and analyzed with the androgen and estrogen yeast bioassay. Results showed that by the introduction of a hydrolysis step, bioassays can be used to screen for the presence of hormone esters in hair samples. Based on the difference in fluorescence responses between the non-hydrolyzed and the hydrolyzed hair samples, it was possible to detect the presence of EB up to at least 56 days after a single pour-on treatment and to detect the presence of TC and TD up to at least 14 days after the treatment. Although the LC-MS/MS analysis could detect TC and TD up to 49 days after treatment, bioassays have the advantage that they can also detect any (un)known steroid ester.

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Authors and Affiliations

  1. Laboratory of Food Analysis, Ghent University, Harelbekestraat 72, 9000, Ghent, Belgium

    Ilse Becue, Christof Van Poucke & Carlos Van Peteghem

  2. RIKILT, Institute of Food Safety, P.O. Box 230, 6700 AE, Wageningen, The Netherlands

    Toine F. H. Bovee, Maria J. Groot & Michel W. F. Nielen

  3. Laboratory of Organic Chemistry, Wageningen University, Dreijenplein 8, 6703 HB, Wageningen, The Netherlands

    Michel W. F. Nielen

Authors
  1. Ilse Becue
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  2. Toine F. H. Bovee
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  3. Christof Van Poucke
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  4. Maria J. Groot
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  5. Michel W. F. Nielen
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  6. Carlos Van Peteghem
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Correspondence to Ilse Becue.

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Becue, I., Bovee, T.F.H., Van Poucke, C. et al. Applicability of a yeast bioassay in the detection of steroid esters in hair. Anal Bioanal Chem 399, 1031–1039 (2011). https://doi.org/10.1007/s00216-010-4401-5

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  • DOI: https://doi.org/10.1007/s00216-010-4401-5

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