Abstract
A 13-min LC–MS method was developed for the determination of daptomycin, a new potent antibiotic, in peritoneal fluid, blood plasma, and urine of patients receiving renal replacement therapy. Chromatography was performed on a C18 column and detection was performed by a single-quadrupole mass spectrometer coupled to LC via an electrospray interface (ESI). The column effluent was also monitored at 370 nm using a photodiode-array detector. The developed method provided a linear dynamic range for concentrations from 0.5 μg mL−1 to 100 μg mL−1. Method precision and accuracy were found to be satisfactory for clinical application, thus the method was successfully used for the analysis of daptomycin in pharmacokinetic studies. The drug was preventively administered against Gram-positive infections to 19 clinical patients undergoing peritoneal dialysis. Peritoneal fluid, blood plasma, and urine samples were collected at 13 time points over a period of 48 h. Clinical samples were analysed following simple sample-preparation procedures and daptomycin was unambiguously detected and quantified.
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Acknowledgment
The authors are grateful to Professor Ian Wilson, Dr Ian Sinclair, and Dr Paul Davey, AstraZeneca, Alderley Park, UK, for their kind donation of LC–MS instrumentation. H. G. Gika is grateful to the EU Commission for a re-integration grant (EIRG 202132) funding her work at the Aristotle University, Thessaloniki.
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Gika, H.G., Michopoulos, F., Divanis, D. et al. Daptomycin determination by liquid chromatography–mass spectrometry in peritoneal fluid, blood plasma, and urine of clinical patients receiving peritoneal dialysis treatment. Anal Bioanal Chem 397, 2191–2197 (2010). https://doi.org/10.1007/s00216-010-3639-2
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DOI: https://doi.org/10.1007/s00216-010-3639-2