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Analysis of hormone antagonists in clinical and municipal wastewater by isotopic dilution liquid chromatography tandem mass spectrometry

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Abstract

A comprehensive method was developed for the simultaneous trace analysis of ten hormone antagonist pharmaceuticals (raloxifene, exemestane, letrozole, anastrozole, mifepristone, finastride, tamoxifen, N-desmethyltamoxifen, clomiphene, and toremifene) in municipal sewage and hospital wastewater samples. The target compounds were firstly extracted using an Oasis HLB cartridge, followed by purification by an aminopropyl cartridge, and were then analyzed by liquid chromatography electrospray ionization tandem mass spectrometry in positive ion mode. The recoveries for the analytes based on internal standard calibration in different test matrices ranged from 67.6 to 118.6% (with the exception of mifepristone in clinical wastewater samples), with relative standard deviations less than 20%. The method quantification limits of the ten pharmaceuticals were in the range 0.10–2.0 ng/L. Excluding exemestane and N-desmethyltamoxifen, eight drugs were detected at 0.20–195.0 ng/L in hospital wastewater and municipal wastewater samples from Beijing.

Analysis of hormone antagonists in clinical and municipal wastewater by liquid chromatography tandem mass spectrometry

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Acknowledgement

The authors gratefully acknowledge financial support from National Natural Science Foundation of China (20837003 and 20607004).

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Authors and Affiliations

  1. Central Laboratory, Beijing Center for Disease Control & Prevention, Beijing, 100013, China

    Xianjun Liu, Jing Zhang, Jie Yin, Hejun Duan & Bing Shao

  2. School of Public Health and Family Medicine, Capital Medical University, Beijing, 100089, China

    Xianjun Liu & Bing Shao

  3. Institute of Nutrition and Food Safety, China Center for Disease Control & Prevention, Beijing, 100021, China

    Yongning Wu

Authors
  1. Xianjun Liu
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  2. Jing Zhang
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  3. Jie Yin
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  4. Hejun Duan
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  5. Yongning Wu
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  6. Bing Shao
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Corresponding author

Correspondence to Bing Shao.

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Liu, X., Zhang, J., Yin, J. et al. Analysis of hormone antagonists in clinical and municipal wastewater by isotopic dilution liquid chromatography tandem mass spectrometry. Anal Bioanal Chem 396, 2977–2985 (2010). https://doi.org/10.1007/s00216-010-3531-0

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  • DOI: https://doi.org/10.1007/s00216-010-3531-0

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