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An immunochemical test for rapid screening of zearalenone and T-2 toxin

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Abstract

An immunochemically based test for non-instrumental simultaneous detection of zearalenone (ZEA) and T-2 toxin (T2) in feed was developed. The method combines clean-up of sample extract, pre-concentration of analytes by immunoextraction and immunodetection through the enzymatic reaction of horseradish peroxidase (HRP). The test is housed inside a standard 1-mL solid-phase extraction column and consists of three layers: two test layers (one for ZEA and another for T2) with immobilised specific antibodies and one control layer with bound anti-HRP antibodies. Feed extract was passed through an additional column with clean-up layer, which was disconnected after extract application. Total assay time was about 15 min for six samples and detection time was 4 min after chromogenic substrate application. Under optimised conditions a cut-off level for ZEA and T2 of 100 µg/kg was established. Different feed types were analysed for ZEA and T2 contamination by the proposed method and results were confirmed by LC-MS/MS.

An immunochemically-based test for non-instrumental simultaneous detection of zearalenone (ZEA) and T-2 toxin (T2) in feed.

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References

  1. JECFA (2001) Joint FAO/WHO expert committee on food additives, 56th meeting. Geneva, Switzerland, 6–15 February 2001

  2. Weidenbörner M (2001) Encyclopedia of food mycotoxins. Springer, Berlin, p 267

    Book  Google Scholar 

  3. Lattanzio VMT, Solfrizzo M, Visconti A (2008) Food Addit Contam 25:320–330

    Article  CAS  Google Scholar 

  4. Schollenberger M, Muller HM, Rufle M, Drochner W (2008) Food Control 19:475–482

    Article  CAS  Google Scholar 

  5. Sulyok M, Berthiller F, Krska R, Schuhmacher R (2007) Anal Bioanal Chem 389:1505–1523

    Article  CAS  Google Scholar 

  6. Sørensen LK, Elbæk TH (2005) J Chromatogr B 820:183–196

    Article  Google Scholar 

  7. Delmulle B, De Saeger S, Adams A, De Kimpe N, Van Peteghem C (2006) Rapid Commun Mass Spectrom 20:771–776

    Article  CAS  Google Scholar 

  8. Schneider E, Curtui V, Seidler C, Dietrich R, Usleber E, Märtlbauer E (2004) Toxicol Lett 153:113–121

    Article  CAS  Google Scholar 

  9. Saha D, Acharya D, Roy D, Shrestha D, Dhar TK (2007) Anal Chim Acta 584:343–349

    Article  CAS  Google Scholar 

  10. Schneider E, Usleber E, Märtlbauer E, Dietrich R, Terplan G (1995) Food Addit Contam 12:387–393

    Article  CAS  Google Scholar 

  11. Abouzied MM, Pestka JJ (1994) J AOAC Int 77:495–501

    CAS  Google Scholar 

  12. Kolosova AYu, De Saeger S, Sibanda L, Verheijen R, Van Peteghem C (2007) Anal Bioanal Chem 389:2103–2107

    Article  CAS  Google Scholar 

  13. Goryacheva IY, De Saeger S, Delmulle B, Lobeau M, Eremin SA, Barna-Vetró I, Van Peteghem C (2007) Anal Chim Acta 590:118–124

    Article  CAS  Google Scholar 

  14. Labuda R, Parich A, Berthiller F, Tancinova D (2005) Intern J Food Microbiol 105:19–25

    Article  CAS  Google Scholar 

  15. Lobeau M, De Saeger S, Sibanda L, Barna-Vetró I, Van Peteghem C (2005) Anal Chim Acta 538:57–61

    Article  CAS  Google Scholar 

  16. Lobeau M, De Saeger S, Sibanda L, Barna-Vetró I, Van Peteghem C (2007) Food Addit Contam 24:398–405

    Article  CAS  Google Scholar 

  17. Goryacheva IYu, De Saeger S, Lobeau M, Eremin SA, Barna-Vetró I, Van Peteghem C (2006) Anal Chim Acta 577:38–45

    Article  CAS  Google Scholar 

  18. Burmistrova NA, Goryacheva IYu, Basova EYu, Franki AS, Elewaut D, Van Beneden K, Deforce D, Van Peteghem C, De Saeger S (2009) Anal Bioanal Chem. doi:10.1007/s00216-009-2913-7

  19. Hack R, Martlbauer E, Terplan G (1989) Lett Appl Microbiol 9:133–135

    Article  CAS  Google Scholar 

  20. Beloglazova NV, Goryacheva IYu, Mikhirev DA, De Saeger S, Niessner R, Knopp D (2008) Anal Sci 24:1613–1617

    Article  CAS  Google Scholar 

  21. Monbaliu S, Van Poucke C, Detavernier C, Dumoulin F, Van De Velde M, Schoeters E, Van Dyck S, Averkieva O, Van Peteghem C, De Saeger S (2009) J Agric Food Chem. doi:10.1021/jf9038592

  22. Basova EY, Goryacheva IY, Mikhirev DA, Rusanova TY, Burmistrova NA, Kerkaert B, Cucu T, De Saeger S, De Meulenauer B (2009) Anal Methods. doi:10.1039/b9ay00144a

  23. Goryacheva IYu, Basova EYu, Van Peteghem C, Eremin SA, Pussemier L, Motte JC, De Saeger S (2008) Anal Bioanal Chem 390:723–727

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors would like to acknowledge the cooperation agreement between Ghent University and Saratov State University. The Belgian federal Science Policy is acknowledged for its financial support; bilateral action; joint Belgian–Chinese project on “Joint study of Fusarium and related mycotoxins in food: detection and control” Bl/02/C46.

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Authors and Affiliations

  1. Chemistry Faculty, Department of Common and Inorganic Chemistry, Saratov State University, Astrakhanskaya 83, 410012, Saratov, Russia

    Evgenia Yu. Basova, Irina Yu. Goryacheva, Tatiana Yu. Rusanova & Natalia A. Burmistrova

  2. Chair of Hygiene and Technology of Milk, Ludwig-Maximilians-Universität München, Schonleutnerstrasse 8, 85764, Oberschleißheim, Germany

    Richard Dietrich & Erwin Märtlbauer

  3. Faculty of Pharmaceutical Sciences, Laboratory of Food Analysis, Ghent University, Harelbekestraat 72, 9000, Ghent, Belgium

    Christ’l Detavernier, Carlos Van Peteghem & Sarah De Saeger

Authors
  1. Evgenia Yu. Basova
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  2. Irina Yu. Goryacheva
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  3. Tatiana Yu. Rusanova
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  4. Natalia A. Burmistrova
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  5. Richard Dietrich
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  6. Erwin Märtlbauer
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  7. Christ’l Detavernier
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  8. Carlos Van Peteghem
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  9. Sarah De Saeger
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Corresponding author

Correspondence to Irina Yu. Goryacheva.

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Basova, E.Y., Goryacheva, I.Y., Rusanova, T.Y. et al. An immunochemical test for rapid screening of zearalenone and T-2 toxin. Anal Bioanal Chem 397, 55–62 (2010). https://doi.org/10.1007/s00216-009-3328-1

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  • DOI: https://doi.org/10.1007/s00216-009-3328-1

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