Abstract
In this paper, we present a surface-plasmon-resonance-based immunosensor for the real-time detection of cortisol and cortisone levels in urine and saliva samples. The method proposed here is simple, rapid, economic, sensitive, robust, and reproducible thanks also to the special features of the polycarboxylate-hydrogel-based coatings used for the antibody immobilization. The sensor surface displays a high level of stability during repeated regeneration and affinity reaction cycles. The immunosensor shows high specificity for cortisol and cortisone; furthermore, no significant interferences from other steroids with a similar chemical structure have been observed. The suitability of the hydrogel coating for the prevention of nonspecific binding is also investigated. A good correlation is noticed between the results obtained by the proposed method and the reference liquid chromatography/tandem mass spectrometry method for the analysis of cortisol and cortisone in urine and saliva samples. Standard curves for the detection of cortisol and cortisone in saliva and urine are characterized by a detection limit less than 10 μg l−1, sufficiently sensitive for both clinical and forensic use.
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White PC, Curnow KM, Pascoe L (1994) Endocr Rev 15:421–438
Purnell JQ, Brandon DD, Isabelle LM, Loriaux DL, Samuels MH (2004) J Clin Endocrinol Metab 89:281–287
Taylor RL, Machacek D, Singh RJ (2002) Clin Chem 48:1511–1519
Orth DN (1995) N Engl J Med 332:791–803
Bobbert T, Brechtel L, Mai K, Otto B, Maser-Gluth C, Pfeiffer AFH, Spranger J, Diederich S (2005) Clin Endocrinol 63:530–536
Lin CL, Wu TJ, Machacek DA, Jiang NS, Kao PC (1997) J Clin Endocrinol Metab 82:151–159
Sherbet DP, Papari-Zareei M, Khan N, Sharma KK, Brandmaier A, Rambally S, Chattopadhyay A, Andersson S, Agarwal AK, Auchus RJ (2007) Molec Cell Endocrinol 265:83–88
Remer T, Maser-Gluth C (2007) Clin Chem 53:1870–1871
Leach C, Alfrey C, Suki W, Leonard J, Rambaut P, Inners L, Smith S, Lane H, Krauhs J (1996) J Appl Physiol 81:105–111
Smith SM, Krauhs JM, Leach CS (1997) Adv Space Biol Med 6:123–128
Pujos E, Flament-Waton MM, Paisse O, Grenier-Loustalot MF (2005) Anal Bioanal Chem 381:244–254
Mirasoli M, Deo SK, Lewis JC, Roda A, Daunert S (2002) Anal Biochem 306:204–211
Knoll W (1998) Annu Rev Phys Chem 49:569–638
Lioubashevski O, Chegel VI, Patolsky F, Katz E, Willner I (2004) J Am Chem Soc 126:7133–7143
Tokareva I, Minko S, Fendler JH, Hutter E (2004) J Am Chem Soc 126:15950–15952
Homola J (2006) Surface plasmon resonance based sensors. Springer, Berlin
Cooper MA (2002) Nat Rev Drug Discovery 1:515–528
Giannetti AM, Koch BD, Browner MF (2008) J Med Chem 51:574–580
Lokate AMC, Beusink JB, Besselink GAC, Pruijn GJM, Schasfoort RBM (2007) J Am Chem Soc 129:14013–14018
Hoa XD, Kirk AG, Tabrizian M (2007) Biosens Bioelectron 23:151–160
Homola J (2008) Chem Rev 108:462–493
Mazzarino M, Botrè F (2006) Rapid Comun Mass Spectrom 20:3465–3476
Mazzarino M, de la Torre X, Botrè F (2008) Anal Bioanal Chem 392:681–698
Morton TA, Myszka DG (1998) Meth Enzymol 295:268–290
Sigal GS, Mrksich M, Whitesides GM (1998) J Am Chem Soc 120:3464–3473
Stenberg E, Persson B, Roos H, Urbaniczky C (1991) J Colloid Interface Sci 143:513–526
Love JC, Estroff LA, Kriebel JK, Nuzzo RG, Withesides GM (2005) Chem Rev 105:1103–1169
Jung LS, Campbell CT, Chinowsky TM, Mar MN, Yee SS (1998) Langmuir 14:5636–5648
Singh N, Husson SM (2006) Langmuir 22:8443–8451
Wei Y, Latour R (2008) Langmuir 24:6021–6029
Myszka DG, He X, Dembo M, Morton TA, Goldstein B (1998) Biophys J 75:583–594
Morton TA, Myszka DG, Chaiken IM (1995) Anal Biochem 227:176–185
Schuck P (1996) Biophys J 70:1230–1249
de Mol NJ, Catalina MI, Fischer MJE, Broutin I, Maier CS, Heck AJR (2004) Biochim Biophys Acta 1700:53–64
Blixt O, Head S, Mondala T, Scanlan C, Huflejt ME, Alvarez R, Bryan MC, Fazio F, Calarese D, Stevens J, Razi N, Stevens DJ, Skehel JJ, van Die I, Burton DR, Wilson IA, Cummings R, Bovin N, Wong C-H, Paulson JC (2004) Proc Natl Acad Sci U S A 101:17033–17038
Linman MJ, Taylor JD, Yu H, Chen X, Cheng Q (2008) Anal Chem 80:4007–4013
Stevens RC, Soelberg SD, Near S, Furlong CE (2008) Anal Chem 80:6747–6751
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Frasconi, M., Mazzarino, M., Botrè, F. et al. Surface plasmon resonance immunosensor for cortisol and cortisone determination. Anal Bioanal Chem 394, 2151–2159 (2009). https://doi.org/10.1007/s00216-009-2914-6
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DOI: https://doi.org/10.1007/s00216-009-2914-6