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Multi-residue analysis of traces of pesticides and antibiotics in honey by HPLC-MS-MS

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Abstract

The aim of this work was to develop an analytical method for simultaneous assay of residues of two families of antibiotics, and three pesticides, in honey. The assays involved a mixture of five tetracyclines, four sulfamides, and the pesticides coumaphos, carbendazim, and amitraz (two metabolites). All the compounds were extracted from honey and pre-concentrated by optimised solid-phase extraction (SPE). Analysis was by high-performance liquid chromatography-mass spectrometry-mass spectrometry (HPLC-MS-MS) using a triple-quadrupole spectrometer in multiple reaction monitoring (MRM) mode in order to identify and quantify the compounds present (Sheth et al J Agric Food Chem 38:1125–1130, 1990). During development of the analytical method a strong matrix effect was found that depended on the floral origin of the honey. This led to the development of a standard additions method to quantify the contaminants sought.

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Authors and Affiliations

  1. Service Central d’Analyse du CNRS, Echangeur de Solaize, BP 22, 69390, Vernaison, France

    Delphine Debayle, Guy Dessalces & Marie Florence Grenier-Loustalot

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  1. Delphine Debayle
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  2. Guy Dessalces
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  3. Marie Florence Grenier-Loustalot
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Correspondence to Guy Dessalces.

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Debayle, D., Dessalces, G. & Grenier-Loustalot, M.F. Multi-residue analysis of traces of pesticides and antibiotics in honey by HPLC-MS-MS. Anal Bioanal Chem 391, 1011–1020 (2008). https://doi.org/10.1007/s00216-008-2003-2

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  • DOI: https://doi.org/10.1007/s00216-008-2003-2

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