Abstract
A simple, quick and nontoxic analytical method for the simultaneous determination of five synthetic antioxidants [t-butyl-4-hydroxyanisole (BHA), 2,6-di-t-butyl-hydroxytoluene (BHT), t-butyl hydroquinone (TBHQ), ethoxyquin (EQ) and 2,6-di-tert-butyl-4-hydroxymethyl-phenol (Ionox 100)] in edible vegetable oil has been developed. The analytes were extracted by ethanol, then separated and detected by GC–MS. Extraction conditions such as volume of ethanol required, mixing time and number of extractions were investigated and optimized by an orthogonal array experimental design. The five compounds behaved linearly in the 0.100∼20.0 mg/L concentration range, and the limits of detection (LOD) for BHA, BHT, TBHQ, EQ and Ionox-100 were 1.00, 0.92, 11.5, 0.83 and 1.39 μg/L, respectively. The recoveries at the tested concentrations of 1.00, 20.0 and 100 mg/kg were 75.6∼123%, with coefficients of variation <10.0%. The proposed procedure was successfully applied to the simultaneous analysis of the five antioxidants in soybean oil, tea oil, edible blended oil, rap oil, peanut oil, peanut blended oil and sesame oil samples purchased from local supermarkets.
Similar content being viewed by others
References
Branen AL (1975) J Am Oil Chem Soc 52:59–63
Dziezak JD (1986) J Food Technol 40:94–102
Hocman G (1988) J Int J Biochem 20:639–651
Williams GM, Iatropoulos MJ (1996) J Cancer Lett 104:49–53
Ling GT (ed) (1989) Handbook of food additives. Chemical Industry Press, Beijing, pp 303–310
Komaitis ME, Kapel M (1985) J Am Oil Chem Soc 62:1371–1372
Viplava-Prasad U, Divakar TE, Hariprasad, K, Prakasa-Sastry, CS (1987) J Food Chem 25:159–164
Kimura S, Terada S (1973) J Food Hyg Soc Japan 14:94–99
Icenhour TP, Van Dolah FM (1991) J Am Oil Chem Soc 68:659–661
Gonzalez M, Gallego M, Valcarcel M (1999) J Chromatogr A 848:529–536
Yang MH, Lin HJ, Choong YM (2002) J Food Res Int 35:627–633
Yankah VV, Ushio H, Ohshima T, Koizumi C (1998) J Lipids 11:1139–1145
Oishi M, Matsuda T, Nojiri S, Saito K (2002) J Shokuhin Eiseigaku Zasshi 43:104–109
Wang H, Liu W (2004) J Sep Sci 27:1189–1194
Gamazo-Vázquez J, García-Falcón MS, Simal-Gándara J (2003) J Food Control 14:463–467
Barrek S, Paisse O, Grenier-Loustalot MF (2003) J Anal Bioanal Chem 376:355–359
Lambropoulou D, Sakellarides T, Albanis T (2000) J Fresenius J Anal Chem 368:616–623
Tombese NB, Freije H (2002) J Chromatogr A 963:179–183
Fries E, Püttmann W (2002) J Water Res 36:2319–2327
Lan WG, Wong MK, Chen N, Sin YM (1995) Analyst 120:1115–1124
Bagheri H, Saraji M, Chitsazan M, Mousavi SR, Naderi M (2000) J Chromatogr A 888:197–208
Zhu GH, Ju HX (2004) Anal Chim Acta 506:177–181
He H, Yu JN, Ni KY (eds) (2004) Modern chromatographic analysis. Chemical Industry Press, Beijing, p 107
Dopico-Garcia MS, Lopez-Vilarino JM, Gonzalez-Rodriguez MV (2005) Talanta 66:1103–1107
Acknowledgements
The financial support for this study from the Program for Changjiang Scholars and Innovative Research Team in University (No: IRT0540), and from the Natural Science Foundation of Jinagxi Province (No.0520057) is gratefully acknowledged.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Guo, L., Xie, MY., Yan, AP. et al. Simultaneous determination of five synthetic antioxidants in edible vegetable oil by GC–MS. Anal Bioanal Chem 386, 1881–1887 (2006). https://doi.org/10.1007/s00216-006-0738-1
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00216-006-0738-1