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Investigations into the electrooxidation of guanosine-5′-triphosphate at the pyrolytic graphite electrode

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Abstract

The electrochemical oxidation of guanosine-5′-triphosphate has been investigated in phosphate-containing electrolytes in the pH range 1.5–10.9 at a pyrolytic graphite electrode by cyclic sweep voltammetry, spectral studies, bulk electrolysis and related techniques. In this pH range, the oxidation occurred in a single well-defined peak (Ia). The peak potential of oxidation peaks (Ep) was found to be dependent on pH, concentration and sweep rate. The kinetics of the UV-absorbing intermediates was followed spectrophotometrically and the decay of the intermediate occurred in a pseudo-first-order reaction. The first-order rate constants for the disappearance of the UV-absorbing intermediate have also been calculated. The products of the electrode reaction were characterized by HPLC and GC/MS. A tentative mechanism for the formation of the products has also been suggested.

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Acknowledgements

One of the authors (A. Tyagi) is thankful to the Council of Scientific and Industrial Research for Junior Research Fellowship. Financial assistance for this work was provided by the CSIR, New Delhi vide Grant No. 01(1815)/02/EMR-II.

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Authors and Affiliations

  1. Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, 247667, India

    Rajendra N. Goyal & Anuradha Tyagi

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  1. Rajendra N. Goyal
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  2. Anuradha Tyagi
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Correspondence to Rajendra N. Goyal.

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Goyal, R.N., Tyagi, A. Investigations into the electrooxidation of guanosine-5′-triphosphate at the pyrolytic graphite electrode. Anal Bioanal Chem 382, 1683–1690 (2005). https://doi.org/10.1007/s00216-005-3344-8

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  • DOI: https://doi.org/10.1007/s00216-005-3344-8

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