Abstract
Methyl salicylate (MeSA) in many plants is a important signaling compound, which plays an important role in a pathogen-induced defense response. In this paper, gas chromatography-mass spectrometry (GC-MS) with headspace solid-phase microextraction (HS-SPME) was developed for determination of MeSA and other volatile compounds in leaves of a tomato plant (Lycopersicon esculentum). Tomato leaves were ground under liquid nitrogen and sampled by HS-SPME, with a 100 μm polydimethylsiloxane fiber, and finally analyzed by GC-MS. Eighteen compounds in the leaves of tomato plant infested by tobacco mosaic virus (TMV) were separated and identified, among them MeSA, which was quantitatively analyzed by the standard addition method. MeSA concentrations higher than 2.0 μg g–1 fresh weight accumulated in leaves of TMV-infested tomato plant as the defense response to TMV. A similar concentration of MeSA in the leaves of MeSA-treated tomato plant was also found. No MeSA in leaves of control tomato plant was detected. These findings suggest that MeSA might be a signaling compound in the tomato plant response to TMV. The present method for determination of MeSA required only simple sample preparation and no organic solvent, and provided an excellent relative standard deviation of less than 5.0% and a low detection limit of 10 ng g−1 fresh weight for MeSA. These results show that GC-MS-HS-SPME is a simple, rapid and sensitive method for determination of MeSA and other plant-signaling compounds in plant tissues.
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References
Gonzalez-Penas E, Lopez-Alvarez M, de Narvajas FM and Ursua A (2000) Chromatographia 52:245–248
Demirci F, Demirci B, Baser KHC, Guven K (2000) Chem Nat Compd 36:159–165
Jayasekara TK, Stevenson PC, Belmain SR, Farman DI, Hall DR (2002) J Mass Spectrom 37:577–580
Lopes D, Koketsu M, Carauta JPP, de Oliveira RR, Kaplan MAC (2002) J Essent Oil Res 14:402–406
Shulaev V, Silverman P, Raskin I (1997) Nature 385:718–721
Seskar M, Shulaev V, Raskin I (1998) Plant Physiol 116:387–392
Zhang XM, Wang L, Zhang J, Kong FM (2001) J Fudan Univ (Nat Sci) 40:375–380
Enyedi AJ, Yalpani N, Silverman P, Raskin I (19992) Proc Natl Acad Sci USA 89:2480–2484
Deng CH, Zhang XM, Zhang J, Qian J, Zhu WM (2003) Chromatographia 58:225–229
Deng CH, Zhang XM, Zhu WM, Qian J (in press) Chin Sci Bull
Arthur CL, Pawliszyn J (1990) Anal Chem 62:2145-2148
Ulrich S (2000) J Chromatogr A 902:167–194
Alpendurada MD (2000) J Chromatogr A 889:3-14
Augusto F, Valente ALP (2002) TrAC Trends Anal Chem 21:428–438
Shang CQ, Hu YM, Deng CH (2002) J Chromatogr. A 942:283–288
Shang CQ, Deng CH, HuYM (2002) Chromatographia 55:737–741
Deng CH, Song GX, Hu YM, Zhang XM (2003) Chromatographia 57:357–361
Deng CH, Song GX, Hu YM, Zhang XM (2003) Chromatographia 57:805–809
Acknowledgements
The study was supported by grants from Natural Science Foundation of China (NSFC20075004) and National Basic Research Priorities Programme (Project No. 0011CB510202).
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Deng, C., Zhang, X., Zhu, W. et al. Gas chromatography-mass spectrometry with solid-phase microextraction method for determination of methyl salicylate and other volatile compounds in leaves of Lycopersicon esculentum . Anal Bioanal Chem 378, 518–522 (2004). https://doi.org/10.1007/s00216-003-2240-3
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DOI: https://doi.org/10.1007/s00216-003-2240-3