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Determination of 4- n -nonylphenol in water by enzyme immunoassay

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Abstract

A range of monoclonal antibody-based competitive immunoassays in the format of microtitre plate ELISA and dipstick tests for quantitative and semi-quantitative detection of 4- n -nonylphenol in water was developed. A simple visual dipstick test was based on changing of spot colour from green to brown in the presence of 4- n -nonylphenol at concentrations within the range 10–100 ng mL−1. Two different detection systems were used for quantitative immunoassay. Application of enhanced chemiluminescence (ECL) resulted in an increase of the sensitivity of ELISA when compared to conventional colorimetric detection. Thus a detection limit of 0.06 ng mL−1 of 4- n -nonylphenol was achieved with IC50 2.0 ng mL−1. The tests developed were applied to natural and spiked water samples.

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Acknowledgements

The authors gratefully acknowledge INTAS (fellowship grant YSF 2001/2–0052) and Russian Ministry of Industry, Science and Technology (project "Analytical nanobiotechnology") for providing financial support.

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Authors and Affiliations

  1. Department of Chemical Enzymology, Chemistry Faculty, M.V. Lomonosov Moscow State University, 119992, Moscow, Russia

    Jeanne V. Samsonova & Maya Yu. Rubtsova

  2. Department of Biotechnology, Veterinary Research Institute, Hudcova 70, 621 32, Brno, Czech Republic

    Milan Franek

Authors
  1. Jeanne V. Samsonova
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  2. Maya Yu. Rubtsova
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  3. Milan Franek
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Correspondence to Jeanne V. Samsonova.

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Samsonova, J.V., Rubtsova, M.Y. & Franek, M. Determination of 4- n -nonylphenol in water by enzyme immunoassay. Anal Bioanal Chem 375, 1017–1019 (2003). https://doi.org/10.1007/s00216-003-1815-3

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  • DOI: https://doi.org/10.1007/s00216-003-1815-3

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