Abstract
The toxicological effects of lead and its compounds have overshadowed its possible health beneficial effects. Currently, the success rate for treating neuropathic pain has been very low. This study investigated the antinociceptive effects of orally administered low dose lead acetate in sciatic nerve ligated Wistar rats. Thirty Wistar rats randomly divided into five groups were used for this study. Chronic constriction injury (CCI) was used to induce neuropathic pain in Wistar rats. Allodynic and hyperalgesic signs were investigated using von Frey filaments and hotplate, respectively. Morris water maze test was used to assess the memory functions of the rats. The study revealed that oral administration of low-dose lead acetate significantly (p < 0.05) increased pain thresholds of ligated rats. CCI enhanced memory function in Wistar rats which was significantly decreased following lead acetate administration. The findings suggest that lead acetate possesses antinociceptive effects in peripherally induced neuropathic pain model in Wistar rats.
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Acknowledgment
Our special appreciation goes to Mr. Muritala Sharafadeen and Bashir Abubakar of the Department of Physiology, University of Ilorin, for their immense technical support during this study.
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No grants were received for this project. It was self-sponsored.
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BVO conceived and designed the research. AOB, KA, DA, and SA conducted experiments and analyzed data. BVO and AOB contributed new reagents and analytical tools. BVO, AOB, and MA supervised the research and wrote the manuscript. All authors read and approved the manuscript and all data were generated in-house and no paper mill was used.
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The study was conducted in accordance with the ARRIVE guidelines (Kilkenny et al. 2013). The protocol was also assessed and approved by the University of Ilorin Ethical Review Committee (UERC/ASN/2019/1744) before the commencement of the research.
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Owoyele, B., Bakare, A., Ayinla, M.T. et al. Antinociceptive effects of lead acetate in sciatic nerve chronic constriction injury model of peripheral neuropathy in male Wistar rats. Naunyn-Schmiedeberg's Arch Pharmacol 394, 117–125 (2021). https://doi.org/10.1007/s00210-020-01951-w
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DOI: https://doi.org/10.1007/s00210-020-01951-w