Abstract
Antiinflammatory properties of pulsed magnetic field (PMF) treatments or administration of antiLy6G antibody have been previously reported. In this study, we hypothesized that, the combination of PMF treatments and antiLy6G administration may synergistically potentiate their antiinflammatory actions. The effects of the combination of PMF treatments and antiLy6G administration were investigated by examining the inflammatory signs, histopathological properties of the inflamed site, and measuring the macrophage inflammatory protein-1 alpha (MIP-1α/CCL3) and myeloperoxidase (MPO) levels of inflamed paw tissues in rats with carrageenan-induced acute paw inflammation. In this present study, PMF treatments alone or administration of antiLy6G alone ameliorated the acute inflammation. However, their combination exacerbated the inflammatory signs, hyperalgesia, allodynia, edema and fever, and aggravated the inflammatory conditions by excessive infiltration of inflammatory cells to the inflamed site. These opposing effects of the combined treatments may correlate with enhanced levels of MIP-1α and MPO in inflamed paws. Present results indicated that the combination of the PMF treatments and antiLy6G administration may not provide additional benefits and may actually cause an aggravation of the acute inflammatory process. Findings may also suggest that during neutrophil or immune cell-targeted treatments for inflammatory states, magnetic field exposure may cause unexpected negative consequences.
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This project was supported by The Scientific and Technological Research Council of Turkey supported the current studies with a project number 116S502.
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Tufan Mert and Selma Yaman designed the project studies and collected behavioral tests and analyzed the data. Tuba Ozcan Metin conducted histopathological examinations and analyzed the results. Mehmet Sahin collected and analyzed the biochemical data. Tufan Mert wrote the paper, and all authors provided useful scientific information contribution into the manuscript. All authors read and approved the manuscript. The authors declare that all experiments were performed in-house and that no paper mill was used.
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In this present study, the experimental protocols were approved by the animal research committee of Kahramanmaras Sutcu Imam University (2016/04/03). All experiments were accomplished in accordance with the guidelines of International Association for the Study of Pain (IASP) Committee for Research and Ethical Issues
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Mert, T., Metin, T.O., Sahin, M. et al. Antiinflammatory properties of antiLy6G antibody disappear during magnetic field exposure in rats with carrageenan induced acute paw inflammation. Naunyn-Schmiedeberg's Arch Pharmacol 393, 2107–2115 (2020). https://doi.org/10.1007/s00210-020-01925-y
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DOI: https://doi.org/10.1007/s00210-020-01925-y