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Evidence of anti-inflammatory activity of Schizandrin A in animal models of acute inflammation

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Abstract

Schisandrin A (Sch A) is a lignin extracted from the fruit of Schisandra chinensis, which has potential anti-inflammatory properties and is used for treating various inflammatory diseases. In this study, we aimed to evaluate the anti-inflammatory effects of Sch A and the underlying mechanisms in animal models of acute inflammation. First, the anti-inflammatory effects of Sch A were evaluated preliminarily in an animal model of xylene-induced ear edema. Sch A pretreatment significantly decreased the degree of edema and inhibited telangiectasia in the ear. Second, a mouse model of paw edema was used to investigate the anti-inflammatory effects and mechanisms of Sch A. Pretreatment with Sch A significantly inhibited carrageenan-induced paw edema in mice. Hematoxylin-eosin (HE) staining of paw tissues demonstrated that Sch A inhibited the infiltration of inflammatory cells in the mouse model of paw edema. Enzyme-linked immunosorbent assay (ELISA) results indicated that the levels of inflammatory factors decreased. The western blot and immunohistochemical assay results revealed that the toll-like receptor 4/nuclear factor kappa-B (TLR4/NF-κB) pathway could play a role in the anti-inflammatory functions of Sch A. The findings demonstrated that Sch A exerts anti-inflammatory effects and may provide possible strategies for the treatment of inflammatory diseases.

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Funding

This research was funded by the Shandong provincial college students innovation and entrepreneurship training program (S201910443039).

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Authors and Affiliations

  1. College of Basic Medicine, Jining Medical University, Jining, 272067, Shandong, China

    Likun Cui, Wenzhe Zhu, Zhijie Yang, Xiyuan Song, Cui Xu & Ziwei Cui

  2. Department of Pathology, Jining No.1 People’s Hospital, Jining, 272011, Shandong, China

    Longquan Xiang

Authors
  1. Likun Cui
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  2. Wenzhe Zhu
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  3. Zhijie Yang
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  4. Xiyuan Song
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  5. Cui Xu
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  6. Ziwei Cui
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  7. Longquan Xiang
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Contributions

LC and LX designed the study. WZ, ZY, and CX performed the experiments. XS and ZC analyzed the data. LC wrote the manuscript.

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Correspondence to Longquan Xiang.

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The authors declare that they have no conflict of interest.

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The Ethics Committee of Jining Medical University approved the experimental protocols (Jining, China).

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Cui, L., Zhu, W., Yang, Z. et al. Evidence of anti-inflammatory activity of Schizandrin A in animal models of acute inflammation. Naunyn-Schmiedeberg's Arch Pharmacol 393, 2221–2229 (2020). https://doi.org/10.1007/s00210-020-01837-x

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  • DOI: https://doi.org/10.1007/s00210-020-01837-x

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