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Suppression of agonist induced Ca2+ oscillations in cultured hepatocytes by nafenopin: possible involvement of protein kinase C

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Abstract

The alpha1-agonist phenylephrine (5 μM) induces an increase in the free cytosolic Ca2+ concentration, followed by repetitive transients of the cytoplasmic Ca2+ concentration, in single Fura-2 loaded hepatocytes. The tumor promoting, hypolipidemic drug nafenopin suppressed the cellular Ca2+ response to phenylephrine. The effect of nafenopin on the Ca2+ increase and Ca2+ oscillations was largely prevented by the specific protein kinase C inhibitor Gö 6976. This finding suggests involvement of protein kinase C in the action of nafenopin on phenylephrine induced Ca2+ mobilization.

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  1. E. Leibold

    Present address: Department of Toxicology, BASF AG, D-67056, Ludwigshafen, Germany

Authors and Affiliations

  1. GSF-Institut für Toxikologie, Postfach 1129, D-85758, Neuherberg/Oberschleissheim, Germany

    E. Leibold, A. Stampfl & L. R. Schwarz

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  1. E. Leibold
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  2. A. Stampfl
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  3. L. R. Schwarz
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Leibold, E., Stampfl, A. & Schwarz, L.R. Suppression of agonist induced Ca2+ oscillations in cultured hepatocytes by nafenopin: possible involvement of protein kinase C. Arch Toxicol 70, 252–255 (1996). https://doi.org/10.1007/s002040050269

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  • DOI: https://doi.org/10.1007/s002040050269

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