Abstract
The effects of the pyrethroid deltamethrin (D) on catecholamine secretion of cultured bovine chromaffin cells were investigated in vitro using high performance liquid chromatography (HPLC). Spontaneous release of catecholamines was increased by 10 μM and 100 μM D. This increase could partially be prevented by the simultaneous use of 2 μM tetrodotoxin (TTX), which reduced the increase by 10 μM D of catecholamine secretion by 90% and that of 100 μM D by 50%. TTX 2 μM alone did not alter the spontaneous release in comparison to controls. Medullary chromaffin cells consist of two cell groups, one secreting mainly epinephrine (E), the other norepinephrine (NE). The ratio between the spontaneously secreted catecholamines E and NE was increased after treatment with D, indicating a dominant effect on E secreting cells.
This is a preview of subscription content, log in via an institution to check access.
References
Brown LD, Narahashi T (1992) Modulation of nerve membrane sodium channel activation by deltamethrin. Brain Res 584: 71–76
Cremer JE, Seville MP (1982) Comparative effects of two pyrethroids, deltamethrin and cismethrin, on plasma catecholamines and on blood glucose and lactate. Toxicol Appl Pharmacol 66: 124–133
Copland RE (1972) The chromaffin system. In: Blaschko H, Muscholl E (eds) Handbook of experimental pharmacology 33. Springer, Berlin, pp 16–45
De Boers SF, Van der Gugten J, Slangen JL, Hijzen TH (1988) Changes in plasma corticosterone and catecholamine contents induced by low doses of deltamethrin in rats. Toxicology 49: 263–270
Elliot M, Farnham AW, Janes NF, Needham PH, Pearson BC (1967) 5-benzyl-3-furylmethyl chrysanthemate: a new potent insecticide. Nature 213: 493–494
Ellis JT, Bandettini PA, Holman PA, Propp JM (1992) Pyrethroid insecticide-induced alterations in mammalian synaptic membrane potential. J Pharmacol Exp Ther 262: 1173–1181
Forshaw PJ, Lister T, Ray DE (1993) Inhibition of neuronal voltage dependent chloride channel by the type II pyrethroid geltamethrin. Neuropharmacology 32: 105–111
Livett BG (1984) Adrenal medullary chromaffin cells in vitro. Physiol Rev 64: 1103–1161
Marxen P, Fuhrmann U, Bigalke H (1989) Gangliosides mediate inhibitory effects of tetanus and botulinum a neurotoxins in chromaffin cells. Toxicon 27: 849–859
Moro MA, Lopez MG, Michelena P, Garcia AG (1990) Seperation and culture of living adrenaline- and noradrenaline-containing cells from bovine adrenal medullae. Anal Biochem 185: 243–248
Müller TH, Unsicker K (1981) High performance liquid chromatografie with electrochemical detection as a highly efficient tool for studying catecholaminergic systems I. Quantification of noradrenaline, adrenaline and dopamine in cultured adrenal medullary cells. J Neurosci Methods 4: 39–52
Narahashi T (1992) Nerve membrane sodium channels as targets of insecticides. Trends Pharmacol Sci 13: 236–241
Vijverberg HPM, Van der Zalm JM, Van den Bercken J (1982) Similar mode of action of pyrethroids and DDT on sodium channel gating in myelinated nerves. Nature 295: 601–603
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bickmeyer, U., Weinsberg, F. & Wiegand, H. Effects of deltamethrin on catecholamine secretion of bovine chromaffin cells. Arch Toxicol 68, 532–534 (1994). https://doi.org/10.1007/s002040050109
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s002040050109