Abstract
To determine the developmental exposure effects of T-2 toxin on postnatal hippocampal neurogenesis, pregnant ICR mice were provided a diet containing T-2 toxin at 0, 1, 3, or 9 ppm from gestation day 6 to day 21 on weaning after delivery. Offspring were maintained through postnatal day (PND) 77 without T-2 toxin exposure. In the hippocampal dentate gyrus of male PND 21 offspring, GFAP+ and BLBP+ type-1 stem cells and PAX6+ and TBR2+ type-2 progenitor cells decreased in the subgranular zone (SGZ) at 9 and ≥3 ppm, respectively, in parallel with increased apoptosis at ≥3 ppm. In the dentate hilus, reelin+ γ-aminobutyric acid (GABA)-ergic interneurons increased at 9 ppm, suggesting reflection of neuronal mismigration. T-2 toxin decreased transcript levels of cholinergic and glutamate receptor subunits (Chrna4, Chrnb2 and Gria2) and glutamate transporter (Slc17a6) in the dentate gyrus, suggesting decreased cholinergic signals on hilar GABAergic interneurons innervating type-2 cells and decreased glutamatergic signals on type-1 and type-2 cells. T-2 toxin decreased SGZ cells expressing stem cell factor (SCF) and increased cells accumulating malondialdehydes. Neurogenesis-related changes disappeared on PND 77, suggesting that T-2 toxin reversibly affects neurogenesis by inducing apoptosis of type-1 and type-2 cells with different threshold levels. Decreased cholinergic and glutamatergic signals may decrease type-2 cells at ≥3 ppm. Additionally, decreased SCF/c-Kit interactions and increased oxidative stress may decrease type-1 and type-2 cells at 9 ppm. The no-observed-adverse-effect level for offspring neurogenesis was determined to be 1 ppm (0.14–0.49 mg/kg body weight/day).
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References
Akane H, Saito F, Yamanaka H, Shiraki A, Imatanaka N, Akahori Y, Morita R, Mitsumori K, Shibutani M (2013) Methacarn as a whole brain fixative for gene and protein expression analyses of specific brain regions in rats. J Toxicol Sci 3:431–443. doi:10.2131/jts.38.431
Arvidsson A, Kokaia Z, Lindvall O (2001) N-methyl-d-aspartate receptor-mediated increase of neurogenesis in adult rat dentate gyrus following stroke. Eur J Neurosci 14:10–18. doi:10.1046/j.0953-816x.2001.01611.x
Cameron HA, McEwen BS, Gould E (1995) Regulation of adult neurogenesis by excitatory input and NMDA receptor activation in the dentate gyrus. J Neurosci 15:4687–4692
Chaudhari M, Jayaraj R, Santhosh SR, Rao PVL (2009a) Oxidative damage and gene expression profile of antioxidant enzymes after T-2 toxin exposure in mice. J Biochem Mol Toxicol 23:212–221. doi:10.1002/jbt.20282
Chaudhari M, Jayaraj R, Bhaskar ASB, Lakshmana Rao PV (2009b) Oxidative stress induction by T-2 toxin causes DNA damage and triggers apoptosis via caspase pathway in human cervical cancer cells. Toxicology 262:153–161. doi:10.1016/j.tox.2009.06.002
Chaudhary M, Rao PV (2010) Brain oxidative stress after dermal and subcutaneous exposure of T-2 toxin in mice. Food Chem Toxicol 48:3436–3442. doi:10.1016/j.fct.2010.09.018
FAO/WHO/UNU (1985) Energy and protein requirements. Report of a joint FAO/WHO/UNU expert consultation. World Health Organ Tech Rep Ser 724:1–206
Fink-Gremmels J (2008) Mycotoxins in cattle feeds and carry-over to dairy milk: a review. Food Addit Contam Part A Chem Anal Control Expo Risk Assess 25:172–180. doi:10.1080/02652030701823142
Fonnum F, Karlsen RL, Malthe-Sørenssen D, Skrede KK, Walaas I (1979) Localization of neurotransmitters, particularly glutamate, in hippocampus, septum, nucleus accumbens and superior colliculus. Prog Brain Res 51:167–191. doi:10.1016/S0079-6123(08)61304-7
Freund TF, Buzsáki G (1996) Interneurons of the hippocampus. Hippocampus 6:347–470. doi:10.1002/(SICI)1098-1063(1996)6:4<347:AID-HIPO1>3.0.CO;2-I
Fujise N, Liu Y, Hori N, Kosaka T (1998) Distribution of calretinin immunoreactivity in the mouse dentate gyrus: II. Mossy cells, with special reference to their dorsoventral difference in calretinin immunoreactivity. Neuroscience 82:181–200. doi:10.1016/S0306-4522(97)00261-3
Gong C, Wang TW, Huang HS, Parent JM (2007) Reelin regulates neuronal progenitor migration in intact and epileptic hippocampus. J Neurosci 27:1803–1811. doi:10.1523/JNEUROSCI.3111-06.2007
Gross A, Jockel J, Wei MC, Korsmeyer SJ (1998) Enforced dimerization of BAX results in its translocation, mitochondrial dysfunction and apoptosis. EMBO J 17:3878–3885. doi:10.1093/emboj/17.14.3878
Harrist A, Beech RD, King SL, Zanardi A, Cleary MA, Caldarone BJ, Eisch A, Zoli M, Picciotto MR (2004) Alteration of hippocampal cell proliferation in mice lacking the beta 2 subunit of the neuronal nicotinic acetylcholine receptor. Synapse 54:200–206. doi:10.1002/syn.20081
Hodge RD, Kowalczyk TD, Wolf SA, Encinas JM, Rippey C, Enikolopov G, Kempermann G, Hevner RF (2008) Intermediate progenitors in adult hippocampal neurogenesis: Tbr2 expression and coordinate regulation of neuronal output. J Neurosci 28:3707–3717. doi:10.1523/JNEUROSCI.4280-07.2008
Holladay SD, Blaylock BL, Comment CE, Heindel JJ, Luster MI (1993) Fetal thymic atrophy after exposure to T-2 toxin: selectivity for lymphoid progenitor cells. Toxicol Appl Pharmacol 121:8–14. doi:10.1006/taap.1993.1122
Hsu IC, Smalley EB, Strong FM, Ribelin WE (1972) Identification of T-2 toxin in moldy corn associated with a lethal toxicosis in dairy cattle. Appl Microbiol 24:684–690
Itahashi M, Wang L, Shiraki A, Abe H, Tanaka T, Murakami T, Yoshida T, Shibutani M (2014) N-methyl-N-nitrosourea during late gestation results in concomitant but reversible progenitor cell reduction and delayed neurogenesis in the hippocampus of rats. Toxicol Lett 226:285–293. doi:10.1016/j.toxlet.2014.02.018
Ito M, Kawa Y, Ono H, Okura M, Baba T, Kubota Y, Nishikawa SI, Mizoguchi M (1999) Removal of stem cell factor or addition of monoclonal anti-c-KIT antibody induces apoptosis in murine melanocyte precursors. J Investig Dermatol 112:796–801. doi:10.1046/j.1523-1747.1999.00552.x
JECFA (2001) Summary and conclusions of the fifty-sixth meeting. Geneva, 6–15 February 2001. http://fao.org/codex/Meetings/CCFAC/ccfac33/56th%20JECFA%20Summary.pdf. Accessed 18 March 2015
Jin K, Mao XO, Sun Y, Xie L, Greenberg DA (2012) Stem cell factor stimulates neurogenesis in vitro and in vivo. J Clin Invest 110:311–319. doi:10.1172/JCI15251
Kaneko T, Fujiyama F, Hioki H (2002) Immunohistochemical localization of candidates for vesicular glutamate transporters in the rat brain. J Comp Neurol 444:39–62. doi:10.1002/cne.10129
Kempermann G, Jessberger S, Steiner B, Kronenberg G (2004) Milestones of neuronal development in the adult hippocampus. Trends Neurosci 27:447–452. doi:10.1016/j.tins.2004.05.013
Lafarge-Frayssinet C, Chakor K, Lafont P, Frayssinet C (1990) Transplacental transfer of T2 toxin: pathological effect. J Environ Pathol Toxicol Oncol 10:64–68
Masiulis I, Yun S, Eisch AJ (2011) The interesting interplay between interneurons and adult hippocampal neurogenesis. Mol Neurobiol 44:287–302. doi:10.1007/s12035-011-8207-z
Middlebrook JL, Leatherman DL (1989) Binding of T-2 toxin to eukaryotic cell ribosomes. Biochem Pharmacol 38:3103–3110. doi:10.1016/0006-2952(89)90021-X
OECD (2007) Guidelines for the Testing of Chemicals, Section 4, Test No. 426: Developmental Neurotoxicity Study. http://www.oecd-ilibrary.org/docserver/download/9742601e.pdf?expires=1432013897&id=id&accnaac=guest&checksum=9E6BE9177018665FA3D81308B242E80F. Accessed 10 May 2015
Ohishi T, Wang L, Ogawa B, Fujisawa K, Taniai E, Hayashi H, Mitsumori K, Shibutani M (2010) No effect of sustained systemic growth retardation on the distribution of reelin-expressing interneurons in the neuron-producing hippocampal dentate gyrus in rats. Reprod Toxicol 30:591–599. doi:10.1016/j.reprotox.2010.08.009
Pujadas L, Gruart A, Bosch C, Delgado L, Teixeira CM, Rossi D, de Lecea L, Martínez A, Delgado-García JM, Soriano E (2010) Reelin regulates postnatal neurogenesis and enhances spine hypertrophy and long-term potentiation. J Neurosci 30:4636–4649. doi:10.1523/JNEUROSCI.5284-09.2010
Qiu S, Weeber EJ (2007) Reelin signaling facilitates maturation of CA1 glutamatergic synapses. J Neurophysiol 97:2312–2321. doi:10.1152/jn.00869.2006
Rafai P, Tuboly S, Bata A, Tilly P, Vanyi A, Papp Z, Jakab L, Tury E (1995) Effect of various levels of T-2 toxin in the immune system of growing pigs. Vet Rec 136:511–514. doi:10.1136/vr.136.20.511
Renzel R, Sadek AR, Chang CH, Gray WP, Seifert G, Steinhäuser C (2013) Polarized distribution of AMPA, but not GABAA, receptors in radial glia-like cells of the adult dentate gyrus. Glia 61:1146–1154. doi:10.1002/glia.22505
Sehata S, Teranishi M, Atsumi F, Uetsuka K, Nakayama H (2003) Doi K T-2 Toxin-induced morphological changes in pregnant rats. J Toxicol Pathol 16:59–65. doi:10.1293/tox.16.59
Sehata S, Kiyosawa N, Makino T, Atsumi F, Ito K, Yamoto T, Teranishi M, Baba Y, Uetsuka K, Nakayama H, Doi K (2004) Morphological and microarray analysis of T-2 toxin-induced rat fetal brain lesion. Food Chem Toxicol 42:1727–1736. doi:10.1016/j.fct.2004.06.006
Shiraki A, Akane H, Ohishi T, Wang L, Morita R, Suzuki K, Mitsumori K, Shibutani M (2012) Similar distribution changes of GABAergic interneuron subpopulations in contrast to the different impact on neurogenesis between developmental and adult-stage hypothyroidism in the hippocampal dentate gyrus in rats. Arch Toxicol 86:1559–1569. doi:10.1007/s00204-012-0846-y
Smith BJ, Holladay SD, Blaylock BL (1994) Hematopoietic alterations after exposure to T-2 mycotoxin. Toxicon 32:1115–1123. doi:10.1016/0041-0101(94)90395-6
Son JH, Winzer-Serhan UH (2008) Expression of neuronal nicotinic acetylcholine receptor subunit mRNAs in rat hippocampal GABAergic interneurons. J Comp Neurol 511:286–299. doi:10.1002/cne.21828
Steiner B, Klempin F, Wang L, Kott M, Kettenmann H, Kempermann G (2006) Type-2 cells as link between glial and neuronal lineage in adult hippocampal neurogenesis. Glia 54:805–814. doi:10.1002/glia.20407
Sun L, Lee J, Fine HA (2004) Neuronally expressed stem cell factor induces neural stem cell migration to areas of brain injury. J Clin Investig 113:1364–1374. doi:10.1172/JCI20001
Tozuka Y, Fukuda S, Namba T, Seki T, Hisatsune T (2005) GABAergic excitation promotes neuronal differentiation in adult hippocampal progenitor cells. Neuron 47:803–815. doi:10.1016/j.neuron.2005.08.023
Wang J, Fitzpatrick DW, Wilson JR (1993) Effect of dietary T-2 toxin on biogenic monoamines in discrete areas of the rat brain. Food Chem Toxicol 31:191–197. doi:10.1016/0278-6915(93)90093-E
Wang L, Ohishi T, Shiraki A, Morita R, Akane H, Ikarashi Y, Mitsumori K, Shibutani M (2012) Developmental exposure to manganese chloride induces sustained aberration of neurogenesis in the hippocampal dentate gyrus of mice. Toxicol Sci 127:508–521. doi:10.1093/toxsci/kfs110
Weidner M, Hüwel S, Ebert F, Schwerdtle T, Galla HJ, Humpf HU (2013) Influence of T-2 and HT-2 toxin on the blood–brain barrier in vitro: new experimental hints for neurotoxic effects. PLoS ONE 8:e60484. doi:10.1371/journal.pone.0060484
WHO (2002) Evaluation of certain mycotoxins in food: fifty-sixth report of the Joint FAO/WHO Expert Committee on Food Additives, WHO Technical Report Series 906, Geneva, Switzerland
Williams PP (1989) Effects of T-2 mycotoxin on gastrointestinal tissues: a review of in vivo and in vitro models. Arch Environ Contam Toxicol 18:374–387. doi:10.1007/BF01062362
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The authors thank Mrs. Shigeko Suzuki for her technical assistance in preparing the histological specimens.
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This work was supported by Health and Labour Sciences Research Grants (Research on Food Safety) from the Ministry of Health, Labour and Welfare of Japan (Grant No. H25-shokuhin-ippan-009).
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Tanaka, T., Abe, H., Kimura, M. et al. Developmental exposure to T-2 toxin reversibly affects postnatal hippocampal neurogenesis and reduces neural stem cells and progenitor cells in mice. Arch Toxicol 90, 2009–2024 (2016). https://doi.org/10.1007/s00204-015-1588-4
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DOI: https://doi.org/10.1007/s00204-015-1588-4