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Effects of synthetic para-nonylphenol isomers administered chronically throughout pregnancy and lactation on reproductive system of mouse pups

  • Reproductive Toxicology
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Abstract

The aim of this study was to analyze the effects of synthetic para-nonylphenol isomers administered chronically throughout pregnancy and lactation on the reproductive system of mouse pups. The two synthetic isomers used in this study showed higher (3E22NP) or lower (44NP) estrogen receptor (ER) binding activity on in vitro yeast assay than a commercial NP (NPmix). Female mice were implanted with a tube filled with one of three NPs and estradiol-17β (E2) before mating. The tube was kept in the mice throughout pregnancy and lactation period, until their pups had weaned at 28 days of age (PND 28). The data indicated that in males, NPs decreased body weight in some dose groups on PND28 and increased weights of testes and epididymides. However, the rate of seminiferous tubules having elongate spermatids (steps10–16) decreased significantly in NPs and E2 treated groups, except for the 44NP groups. In female mice, NPs and E2 increased weights of ovaries, uterus and vagina in the pups in some dose groups on PND 28. However, there were no differences in the day of vaginal opening and numbers of corpus lutea. The present study demonstrates that the effects of these two isomers of NP on the pup reproduction at PND 28 are not quite different, despite them showing different levels of ER binding activity using in vitro yeast assay.

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Acknowledgments

This study was supported in part by a Grant-in aid for the Effective Promotion of Joint Research with Industry, Academia, and Government, the Special Coordination Funds for Promoting Science and Technology from the Ministry of Education, Science, Sports and Culture in Japan.

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Authors and Affiliations

  1. College of Bioresource Sciences, Nihon University, Fujisawa, Japan

    Tamio Sato, Takao Katase & Osamu Kai

  2. College of Pharmacy, Nihon University, Funabashi, Japan

    Hiroaki Saito & Taketo Uchiyama

  3. College of Humanities and Sciences, Nihon University, Tokyo, Japan

    Yasuo Fujimoto

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  1. Tamio Sato
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  2. Hiroaki Saito
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  3. Taketo Uchiyama
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  4. Yasuo Fujimoto
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  5. Takao Katase
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  6. Osamu Kai
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Correspondence to Osamu Kai.

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Sato, T., Saito, H., Uchiyama, T. et al. Effects of synthetic para-nonylphenol isomers administered chronically throughout pregnancy and lactation on reproductive system of mouse pups. Arch Toxicol 83, 1097–1108 (2009). https://doi.org/10.1007/s00204-009-0464-5

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