Abstract
When isolated human lymphocytes were treated in vitro with various concentrations of soluble form of nickel carbonate hydroxide (NiCH) (0–1 mM), at 37°C for 4 h, both concentration- and time-dependent effects of NiCH on lymphocyte death were observed. Increased generation of hydrogen peroxide (H2O2), superoxide anion (O −2 ), depletion of both no protein (NP-) and protein (P-) sulfhydryl (SH) contents and lipid peroxidation (LPO) were induced by NiCH. Pretreatment of lymphocytes with either catalase (H2O2 scavenger), or deferoxamine (DFO) (iron chelator), or excess glutathione (GSH) (an antioxidant) not only significantly reduced the NiCH-induced generation of H2O2 and LPO, but also increased the NP-SH and P-SH contents initially reduced by NiCH. NiCH-induced generation of excess O −2 but not excess LPO was significantly reduced by pretreatment with superoxide dismutase (SOD). NiCH-induced lymphocyte death was significantly prevented by pre-treatment with either catalase, or dimethylthiourea/mannitol (hydroxyl radical scavengers), or DFO, or excess GSH/N-acetylcysteine. NiCH-induced lymphocyte death was also significantly prevented by pretreatment with excess SOD. Thus, various types of oxidative stresses play an important role in NiCH-induced lymphocyte death. Cotreatment with cyclosporin A (a specific inhibitor of alteration in mitochondrial membrane potential (ΔΨm) not only inhibited NiCH-induced alteration in ΔΨm, but also significantly prevented Ni-compound-induced lymphocyte death. Furthermore, NiCH-induced destabilization of cellular calcium homeostasis. As such, NiCH-induced lymphocyte death was significantly prevented by modulating intracellular calcium fluxes such as Ca2+ channel blockers and intracellular Ca2+ antagonist. Thus, the mechanism of NiCH (soluble form)-induced activation of lymphocyte death signalling pathways involves not only the excess generation of different types of oxidative stress, but also the induction of alteration in ΔΨm and destabilization of cellular calcium homeostasis as well.
Similar content being viewed by others
Abbreviations
- NiCH:
-
Nickel carbonate hydroxide
- GSH:
-
Glutathione
- DFO:
-
Deferoxamine
- DMTU:
-
Dimethylthiourea
- NP-SH:
-
No protein sulfhydryl
- P-SH:
-
Protein sulfhydryl
- SOD:
-
Superoxide dismutase
- LPO:
-
Lipid peroxidation
- ΔΨm :
-
Mitochondrial membrane potential
- [Ca2+]i :
-
Free intracellular calcium level
References
Antico A, Soana R (1999) Chronic allergic-like dermatopathies in nickel-sensitive patients. Results of dietary restrictions and challenge with nickel salts. Allergy Asthma Proc 20:235–242
Arsalane K, Aerts C, Wallaert B, Voisin C, Hildebrand HF (1992) Effect of nickel hydroxycarbonate on alveolar macrophage functions. J Appl Toxicol 12:285–290
Arsalane K, Hildebrand HF, Martinez R, Wallaert B, Voisin C (1994) Ultrastructural and biochemical changes in alveolar macrophages exposed to nickel hydroxyl carbonate. Sci Total Environ 148:175–183
Boyum A (1976) Isolation of lymphocytes, granulocytes and macrophages. Scand J Immunol 5(Suppl 5):9–15
Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 248:248–254
Chakrabarti SK, Bai C, Subramanian KS (1999) DNA-protein crosslinks induced by nickel compounds in isolated rat renal cortical cells and its antagonism by specific amino acids and magnesium ion. Toxicol Appl Pharmacol 154:245–255
Chakrabarti SK, Bai C, Subramanian KS (2001) DNA-protein crosslinks induced by nickel compounds in isolated rat lymphocytes: role of reactive oxygen species and specific amino acids. Toxicol Appl Pharmacol 170:153–165
Chen C-Y, Wang Y-F, Lin Y-H, Yen S-F (2003) Nickel induced oxidative stress and effect of antioxidants in human lymphocytes. Arch Toxicol 77:123–130
Chen C-Y, Su YJ, Wu PF, Shyu MM (2002) Nickel-induced plasma lipid peroxidation and effect of antioxidants in human blood: involvement of hydroxyl radical formation and depletion of α-tocopherol. J Toxicol Environ Health 65:843–852
Ciccarelli RB, Hampton TH, Jennette KW (1981) Nickel carbonate induces DNA-protein crosslinks and DNA strand breaks in rat kidney. Caner Lett 12:349–354
Ciccarelli RB, Wetterhahn KE (1982) Nickel distribution and DNA lesions induced in rat tissues by the carcinogen nickel carbonate. Cancer Res 42:3544–3549
Ciccarelli RB, Wetterhahn KE (1984) Molecular basis for the activity of nickel. In: Sunderman FW Jr (ed) Nickel in the Human Environment. International Agency for Research on Cancer, Lyon, pp 201–213
Crompton M (1999) The mitochondrial permeability transition pore and its role in cell death. Biochem J 341:233–249
Denkhaus E, Salnikow K (2002) Nickel essentiality, toxicity and carcinogenicity. Crit Revs Oncol/Hematol 42:35–56
Doll R (ed.) (1990) Report of the international committee on Nickel carcinogenesis in man. Scand J Work Environ Health 16:1–82
Dotson RL (1972) Characterization and studies of some four, five and six coordinate transition and representative metal complexes of tris-(hydroxymethyl)-aminomethane. J Inorg Nucl Chem 34:3131–3138
Egedahl RD, Coppock E, Homik R (1991) Mortality experience at a hydrometallurgical nickel refinery in Fort Saskatchewan, Alberta between 1954 and 1984. J Soc Occup Med 41:29–33
Faurskov B, Bjerregard HF (2002) Evidence for cadmium mobilization of intracellular calcium through a divalent cation receptor in renal distal epithelial A6 cells. Pflugers Arch-Eur J Physiol 445:40–50
Funakoshi T, Kuromatsu K, Kojima S (1996) Effect of nickel on enzymatic activities in the mouse pancreas. Res Commun Mol Pathol Pharmacol 92:245–252
Funakoshi T, Inoue T, Shimada H, Kojima S (1997) The mechanism of nickel uptake by rat primary hepatocyte cultures: role of calcium channels. Toxicology 124:21–26
Graf E, Penniston JT (1980) Method for determination of hydrogen peroxide, with its application illustrated by glucose assay. Clin Chem 26(5):658–660
Grynkiewicz G, Poenie M, Tsien R (1985) A new generation of Ca2+ indicators with greatly improved fluorescence properties. J Biol Chem 260:3440–3450
Herlant-Peers MC, Hildebrand HF, Kerckaert JP (1983) In vitro and in vivo incorporation of 63Ni(II) into lung and liver subcellular farctions of Balb/C mice. Carcinogenesis 4:387–392
Hildebrand HF, Decaestecker AM, Hetuin D (1987) Binding of nickel sulfides to lymphocyte subcellular structures. In: trace elements in human health and disease. WHO-CEC-EPA Environmental Health Series, vol 20, pp 82–85
Huang X, Frenkel K, Klein CB, Costa M (1993) Nickel induces increased oxidants in intact cultured mammalian cells as detected by dichlorofluorescein fluorescence. Toxicol Appl Pharmacol 120:29–36
Kasprzak KS (1991) The role of oxidative damage in metal carcinogenicity. Chem Res Toxicol 4:604–615
Kasprzak KS, Sunderman FW Jr, Salnikow K (2003) Nickel carcinogenesis. Mutation Res 533:67–97
Kawanishi S, Oikawa S, Inoue S, Nishino K (2002) Distinct mechanisms of oxidative damage induced by carcinogenic nickel subsulfide and nickel oxides. Environ Health Perspect 110(Suppl.5):789–791
Klein CB, Frenkel K, Costa M (1991) The role of oxidative processes in metal carcinogenesis. Chem Res Toxicol 4:592–604
Lee JE, Ciccarelli RB, Jennette KW (1982) Solubilization of the carcinogen nickel subsulfide and its interaction with deoxyribonucleic acid and protein. Biochemistry 21:771–778
Lemasters JJ (1998) The mitochondrial permeability transition: from biochemical curiosity to pathophysiological mechanism. Gastroenterology 115:783–786
Lemasters JJ, Nieminen AL, Qian T, Trost LC, Elmore S, Nishimura Y, Crowe RA et al (1998) The mitochondrial permeability transition in cell death: a common mechanism in necrosis, apoptosis and autophasy. Biochim Biophys Acta 1366:177–196
M’Bemba-Meka P, Chakrabarti SK (2001) Effects of different nickel compounds on the transport of para-aminohippurate ion by rat renal cortical slices. Toxicol Lett 122:235–244
M’Bemba-Meka P, Lemieux N, Chakrabarti SK (2005) Role of oxidative stress, mitochondrial membrane potential, and calcium homeostasis in nickel sulfate-induced human lymphocyte death in vitro. Chem Biol Interact 156:69–80
Myslak M, Kosmider K (1997) Frequency of sister chromatid exchanges (SCE) in peripheral blood lymphocytes from stainless steel welders. Med Pr 48:399–406
Nackerdien Z, Kasprzak KS, Rao G, Halliwell B, Dizdaroglu M (1991) Nickel(II)- and Cobalt(II)-dependent damage by hydrogen peroxide to the DNA bases in isolated human chromatin. Cancer Res 51:5837–5842
Nieober E, Nriagu JO (eds) (1992) Nickel and human health: current perspectives. Wiley, New York
Obone E, Chakrabarti SK Bai C, Malick MA (1999) Toxicity and bioaccumulation of nickel sulfate in Sprague–Dawley rats following 13 weeks of subchronic exposure. J Toxicol Environ Health 56:101–123
Perminova IN, Sinel’shchikova TA, Alekhina NI, Perminova EV, Zasukhina GD (2001). Individual sensitivity to genotoxic effects of nickel and antimutagenic activity of ascorbic acid. Bull Exp Biol Med 131:367–370
Rauen U, Petrat F, Li T, De Groot H (2000) Hypothermia injury/cold-induced apoptosis–evidence of an increase in chelatable iron causing oxidative injury in spite of low O −2 /H2O2 formation. FASEB J 14:1953–1964
Refvik T, Andreassen T (1995) Surface binding and uptake of Nickel(II) in human epithelial kidney cells: modulation by ionomycin, nicardipine and metals. Carcinogenesis 16:1107–1112
Riley MR, Boesewetter AM, Sirvent FP (2003) Effects of metals Cu, Fe, Ni, V, and Zn on rat lung epithelial cells. Toxicology 190:171–184
Rosen LB, Ginty DD, Greenberg ME (1995) Calcium regulation of gene expression. Adv Second Messenger Phosphoprotein res 30:225–253
Ruch W, Cooper PH, Baggiolini M (1983) Assay of H2O2 production by macrophages and neutrophils with homovanilic acid and horseradish peroxidase. J Immunol Methods 63:347–357
Salnikow K, Kluz T, Costa M (1999) Role of Ca2+ in the regulation of nickel-inducible Cap43 gene expression. Toxicol Appl Pharmacol 160:127–132
Schnellman RG (1988) Mechanisms of t-butyl hydroperoxide-induced toxicity to rabbit renal proximal tubules. Am J Physiol 255:C28–C33
Sedlak J, Lindsay RH (1968) Estimation of total, protein-bound, and nonprotein sulfhydryle groups in tissue with Ellman’s reagent. Anal Biochem 25:192–205
Shirali P, Hildebrand HF, Decaestecker AM, Bailly C, Henichart JP, Martinez R (1992) Interaction of Ni3S2 with plasma membrane of lung cells. In: Nieboer E, Nriagu JO (eds) Nickel and Human health: Current perspectives. Wiley, New York, pp 343–352
Smith JB, Dwyer SD, Smith L (1989) Cadmium evokes inositol polyphosphate formation and calcium mobilization. Evidence for a cell surface receptor that cadmium stimulates and zinc antagonizes. J Biol Chem 264:7115–7118
Sunderman FW Jr (1988) Nickel. In: Sigel H (ed) Handbook on toxicity of inorganic compounds. Marcel Dekker, New York, pp 454–468
Swierenga SHH, Whitefield JF, Gillan DJ (1976) Alteration by malignant transformation of the calcium requirements for cell proliferation in vitro. J Natl Cancer Inst 57:125–129
Torreilles J, Guérin M-C (1990) Ni(II) as a temporary catalyst for hydroxyl radical generation. FEBS 272:58–60
Trump FB, Berezesky KI, Smith MW, Phelps PC, Elliget KA (1989) The relationship between cellular ion deregulation and acute and chronic toxicity. Toxicol Appl Pharmacol 97:6–22
Werfel U, Langen V, Eickhoff I, Schoonbrood J, Vahrenholz C, Brauksiepe A, Popp W, Norpoth K (1998) Elevated DNA single-strand breakage frequencies in lymphocytes of welders exposed to chromium and nickel. Carcinogenesis 19:413–418
Wozniak K, Blasiak J (2002) Free radicals-mediated induction of oxidized DNA bases and DNA-protein crosslinks by nickel chloride. Mutat Res 514:233–243
Wu E., Smith MT, Bellomo G, Di Monte D (1990) Relationships between the mitochondrial transmembrane potential, ATP concentration, and cytotoxicity in isolated rat hepatocytes. Arch Biochem Biophys 282:358–362
Zamponi GW, Bourinet E, Snutch TP (1996) Nickel block of a family of neuronal calcium channels: subtype- and subunit-dependent action at multiple sites. J Membrane Biol 151:77–90
Acknowledgements
The authors sincerely thank Sainte-Justine Hospital, for obtaining blood samples from donors and from the laboratory of vascular immunology of Dr. Genevieve Rainier and Dr. Jean-Claude Mamputu of CHUM, Research Center, Notre-Dame Hospital, Montreal.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
M’Bemba-Meka, P., Lemieux, N. & Chakrabarti, S.K. Role of oxidative stress, mitochondrial membrane potential, and calcium homeostasis in human lymphocyte death induced by nickel carbonate hydroxide in vitro. Arch Toxicol 80, 405–420 (2006). https://doi.org/10.1007/s00204-006-0060-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00204-006-0060-x