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Consecutive administration of paraquat to rats induces enhanced cholesterol peroxidation and lung injury

  • Organ Toxicity and Metabolisms
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Abstract

It is our hypothesis that as a consequence of increased oxidative stress, rats develop lung injury with increased cholesterol-derived hydroperoxides and oxysterols in lung after consecutive exposure of the rats to paraquat. To test this we administered 10 mg/kg of paraquat i.p. once or seven times (once a day) to Wistar rats. Rats were killed, and lung tissue was collected 24 h after the last paraquat injection. We found that in response to consecutive paraquat doses, there were significant increases in 7α- and 7β-hydroperoxycholest-5-en-3β-ol (7α-OOH and 7β-OOH; P=0.01) as well as 7α- and 7β-hydroxycholesterol (7α-OH and 7β-OH; P=0.01), and 7-ketocholesterol (7-keto; P=0.03). In addition, pulmonary hemorrhage, thickening of alveolar septum, and inflammatory cell infiltration of macrophages were observed. This is the first report showing enhanced cholesterol peroxidation and lung injury of rats due to consecutive doses of paraquat.

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Acknowledgements

The authors wish to thank Ms Toshiko Okuyama for her technical assistance. This work was supported in part by a Grant-in-aid for Scientific Research from the Japan Society for the Promotion of Science.

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Authors and Affiliations

  1. Department of Legal Medicine, Kobe University Graduate School of Medicine, 7 Kusunoki-cho, Chuo-ku, 650-0017, Kobe, Japan

    Junko Adachi, Kenji Ishii, Tetsuo Fujita, Yudha Nurhantari & Yasuiro Ueno

  2. Kawasaki Medical School, Kurasiki, Japan

    Masafumi Tomita

  3. Medical Examiner's Office of Hyogo Prefecture, Kobe, Japan

    Yasushi Nagasaki

Authors
  1. Junko Adachi
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  2. Kenji Ishii
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  3. Masafumi Tomita
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  4. Tetsuo Fujita
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  5. Yudha Nurhantari
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  6. Yasushi Nagasaki
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  7. Yasuiro Ueno
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Correspondence to Junko Adachi.

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Adachi, J., Ishii, K., Tomita, M. et al. Consecutive administration of paraquat to rats induces enhanced cholesterol peroxidation and lung injury. Arch Toxicol 77, 353–357 (2003). https://doi.org/10.1007/s00204-003-0449-8

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  • DOI: https://doi.org/10.1007/s00204-003-0449-8

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