High rate of aerobic nitrification and denitrification by Nitrosomonas eutropha grown in a fermentor with complete biomass retention in the presence of gaseous NO2 or NO

Abstract

A pure culture of the obligately lithoautotrophic ammonia-oxidizer Nitrosomonas eutropha was grown in a laboratory-scale bioreactor with complete biomass retention. The air supply was supplemented with nitrogen dioxide (NO₂; 25 or 50 ppm) or nitric oxide (NO; 25 or 50 ppm). Compared to cultures grown without these nitrogenous oxides, the addition of NO₂ or NO to the culture resulted in a significant increase of the nitrification rate, specific activity of ammonia oxidation, growth rate, and maximum cell densities. In contrast, the growth yield slightly decreased in the presence of NO or NO₂. Maximum cell densities of about 2 × 10¹⁰ cells ml^–1 and a maximum nitrification rate of about 221 mmol NH₄ ⁺ l^–1 day^–1 were obtained after 3 weeks in the presence of 50 ppm NO₂. Furthermore, in the stationary phase about 50% of the nitrite produced was aerobically denitrified to dinitrogen (N₂) and traces of nitrous oxide (N₂O). When cells were supplemented with NO, a high rate of aerobic denitrification occurred only during the first days of the exponential growth phase.