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Evidence that two genomic species of Rhizobium are associated with Medicago truncatula

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Abstract

Seventy-three isolates of rhizobia sampled from root nodules of Medicago truncatula were analyzed by restriction fragment length polymorphism (RFLP) of DNA regions amplified by the polymerase chain reaction (PCR) targeting the symbiotic plasmid (nifD-K, nodD1, and nodD2 genes) and the chromosome (16S rDNA plus intergenic spacer). Two genotypic groups were found, regardless of the DNA region targeted. These two groups were given the status of genomic species based on results of DNA/DNA hybridization.

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Authors and Affiliations

  1. Laboratoire d'Ecologie Microbienne du Sol, URA-CNRS 1977, Université Lyon I, Bât 741, 43 Bd du 11 Novembre 1918, F-69622 Villeurbanne, Cedex, France Fax: +33-72-431223 e-mail: sr@geometrie.univ-lyon1.fr, , , , , , FR

    Sophie Rome, Philippe Normand & Maria Fernandez

  2. Laboratoire de Recherche sur les Symbiotes des Racines, ENSAM-INRA, 2 Place P. Viala, F-34060 Montpellier, Cedex 01, France , , , , , , FR

    Brigitte Brunel & Jean-Claude Cleyet-Marel

Authors
  1. Sophie Rome
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  2. Brigitte Brunel
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  3. Philippe Normand
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  4. Maria Fernandez
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  5. Jean-Claude Cleyet-Marel
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Received: 1 August 1995 / Accepted: 13 October 1995

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Rome, S., Brunel, B., Normand, P. et al. Evidence that two genomic species of Rhizobium are associated with Medicago truncatula. Arch Microbiol 165, 285–288 (1996). https://doi.org/10.1007/s002030050328

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  • DOI: https://doi.org/10.1007/s002030050328

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