Abstract
A Gram-staining-positive, heterotrophic, non-spore-forming, non-motile, rod-shaped, strain ZS14-85T belonging to the genus Cryobacterium was isolated from soil in Antarctica. Growth was observed in the presence of 0–2% (w/v) NaCl, at pH 7.0–9.0 (optimum, pH 7.0) and 4–30 ℃ (optimum, 20 ℃). Phylogenetic analysis showed that strain ZS14-85T formed a lineage in the genus Cryobacterium. The digital DNA-DNA hybridization (dDDH) values between strain ZS14-85T and its close relatives Cryobacterium psychrotolerans CGMCC 1.5382T, Cryobacterium soli MCCC 1K03549T and Cryobacterium breve NBRC 113800T were 22.5, 22.3 and 22.2%, respectively. Orthologous Average Nucleotide Identity (OrthoANI) scores between strain ZS14-85T and C. psychrotolerans CGMCC 1.5382T, C. breve NBRC 113800T and C. soli MCCC 1K03549T were 78.7, 78.1 and 77.7%, respectively. The polar lipids of strain ZS14-85T were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), one unidentified glycolipid (GL) and two unidentified lipids (L). The major fatty acids were anteiso-C15:0 (60.7%), iso-C16:0 (17.0%) and anteiso-C17:0 (15.2%). MK-10, MK-11 and MK-9 were the predominant respiratory menaquinones. Based on phenotypic, phylogenetic and genotypic data, a novel species, Cryobacterium zhongshanensis sp. nov. is proposed. The type strain is ZS14-85T (= CCTCC AB 2019396T = KCTC 49384T).
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Data availability
The whole genome sequence of Cryobacterium zhongshanensis ZS14-85T has been deposited in the NCBI GenBank database under the accession number JALGAR000000000. The GenBank accession number of the 16S rRNA gene sequence of strain ZS14-85T is MN637799.
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This work was supported by the National Key R&D Program of China (grants 2022YFC2807500).
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GJN and WJW carried out the data analysis and wrote the manuscript. GJN, HMM and PQJ performed the experiments. XTR and JL participated in the data analysis. JL supervised the project. All authors reviewed and approved the final manuscript.
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Niu, G., Wang, W., Mu, H. et al. Cryobacterium zhongshanensis sp. nov., an actinobacterium isolated from Antarctic soil. Arch Microbiol 205, 79 (2023). https://doi.org/10.1007/s00203-023-03408-z
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DOI: https://doi.org/10.1007/s00203-023-03408-z