Abstract
A Gram-stain-negative, non-motile and coccoid bacterial strain, designated XHP0099T, was isolated from the coastal water of the Yellow Sea, China. Growth occurred at 20–37 ℃ (optimum, 28 ℃), pH 5.0–9.0 (optimum, pH 7.0–8.0), and with 0–7.0% NaCl (optimum, 2.0–3.0%). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain XHP0099T was related to members of the genus Paracoccus and shared the highest sequence similarity with “P. siganidrum” M26 (98.2%), followed by P. alkanivorans 4-2 T (97.6%) and P. alkenifer DSM 11593 T (97.4%). The average nucleotide identity, amino acid identity, and digital DNA–DNA hybridization values of strain XHP0099T against related members in the genus Paracoccus were below the cut-off points proposed for the delineation of a novel species. The major cellular fatty acids (> 10%) were summed feature 8 (C18:1 ω7c/C18:1 ω6c), and C18:0. The major isoprenoid quinone was Q-10 and the polar lipids contained diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylcholine (PC), aminolipid (AL) and unidentified polar lipids (L). The G + C content of the genomic DNA of strain XHP0099T was 66.0%. Genomic analysis suggested that strain XHP0099T harbored gene clusters for formaldehyde and the XoxF-type methanol oxidation and type 1 Calvin cycle, which could confer the methylotrophy pathway. Based on the phenotypic, phylogenetic, biochemical and chemotaxonomic analysis, strain XHP0099T represents a novel species of the genus Paracoccus, for which the name Paracoccus marinaquae sp. nov. is proposed. The type strain is XHP0099T (= JCM 34661 T = GDMCC 1.2414 T = MCCC 1K05846T).
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Data availability
The GenBank/EMBL/DDBJ accession numbers of strain XHP0099T are ON329670 (16S rRNA gene) and JAHKNG000000000 (genome), respectively. Other datasets generated and analyzed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
This research was supported by the National Natural Science Foundation of China (No. 31900001), the Innovation Project for Marine Science and Technology of Jiangsu Province (JSZRHYKJ202209), and the Fundamental Research Funds for the Central Universities (B210202140).
Funding
National Natural Science Foundation of China (No. 31900001). Innovation Project for Marine Science and Technology of Jiangsu Province (JSZRHYKJ202209). Fundamental Research Funds for the Central Universities (B210202140).
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HPX and DFZ: designed research and project outline. LW: provided the three type strains. HPX: performed isolation, deposition and polyphasic taxonomy. HPX, and ZYF: performed genome analysis. ZYF: drafted the manuscript. DFZ, WH, WJL, AHZ, JKH and CL: revised the manuscript. All authors read and approved the final manuscript.
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Xue, HP., Fu, ZY., He, W. et al. Paracoccus marinaquae sp. nov., isolated from coastal water of the Yellow Sea. Arch Microbiol 205, 58 (2023). https://doi.org/10.1007/s00203-023-03402-5
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DOI: https://doi.org/10.1007/s00203-023-03402-5