Abstract
The growth of Phytophthora capsica, Rhizoctonia solani, Fusarium graminearum, Fusarium oxysporum and Botrytis cinerea were all inhibited by the fermentation supernatant of Bacillus licheniformis TG116, a biocontrol strain isolated from Typhonium giganteum Engl. previously with broad-spectrum resistance to plant pathogens. The fermentation supernatant of the TG116 has a great stability on temperature and UV, and shows the biological activity of protease and cellulase. The antifungal protease produced by B. licheniformis TG116 was purified to homogeneity by ammonium sulfate precipitation, DEAE Sepharose Fast Flow column chromatography and Sephadex G-50 column chromatography. The inhibition of protease by the three surfactants increased with increasing concentration inhibition. Among these surfactants, EDTA showed the strongest inhibition, with only 25% protein activity at a concentration of 1.1 mmol·L−1. Gene amplification verified the presence of a gene fragment of serine protease in the strain TG116. The antimicrobial substance isolated from the fermentation broth of TG116 is a serine protease component.
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The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
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Funding
This work was supported by Lanzhou Science and Technology Plan Project (2018–1-104); Special Fund Project for Guiding Science and Technology Innovation and Development in Gansu Province (2019ZX-05); Industrial support Plan Project of Colleges and Universities in Gansu Province (2020C-21).
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LL: Put forward experimental ideas and provide funds; WC, KJ: Complete the experiment and write the first draft of the paper; ZJ, HL, CY, MP: The processing of Experimental data and the making of related Chart; LL: revise the manuscript. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Communicated by Erko Stackebrandt.
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Ling, L., Cheng, W., Jiang, K. et al. The antifungal activity of a serine protease and the enzyme production of characteristics of Bacillus licheniformis TG116. Arch Microbiol 204, 601 (2022). https://doi.org/10.1007/s00203-022-03216-x
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DOI: https://doi.org/10.1007/s00203-022-03216-x