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Mesonia aestuariivivens sp. nov., isolated from a tidal flat

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Abstract

A Gram-negative, aerobic, non-flagellated and ovoid or rod-shaped bacterial strain (JHPTF-M18T), which was isolated from a tidal flat sediment in Republic of Korea, was taxonomically characterized. Strain JHPTF-M18T grew optimally at 25 °C, at pH 7.0–7.5 and in the presence of 2.0–3.0% (w/v) NaCl. 16S rRNA gene sequence analysis showed that strain JHPTF-M18T forms a phylogenetic lineage within the radiation comprising type strains of Mesonia species. The 16S rRNA gene of strain JHPTF-M18T shared sequence similarities of 97.7% with that of type strain of M. mobilis and 92.5–96.8% with those of type strains of the other nine Mesonia species. The DNA G+C content was 33.1% based on its genomic sequence. AAI, ANI and dDDH values between strain JHPTF-M18T and the type strains of M. mobilis, M. hitae, M. oceanica, M. phycicola and M. algae were 72.1–83.7%, 73.1–79.7% and 18.5–22.8%, respectively. Strain JHPTF-M18T contained MK-6 as the predominant menaquinone and iso-C15:0, iso-C17:0 3-OH and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c) as its major fatty acids. Major polar lipids of strain JHPTF-M18T were phosphatidylethanolamine and two unidentified lipids. Strain JHPTF-M18T was separated from recognized Mesonia species by its phenotypic properties together with the phylogenetic and genetic distinctiveness. Based on data presented in this study, strain JHPTF-M18T is considered to represent a novel species of the genus Mesonia. The name Mesonia aestuariivivens sp. nov. is proposed for JHPTF-M18T (=KACC 22185T = NBRC 115119T).

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Abbreviations

ANI:

Average nucleotide identity

AAI:

Average amino acid identity

dDDH:

Digital DNA–DNA hybridization

GC:

Gas chromatography

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Funding

This work was supported by the project on survey of indigenous species of Korea of the National Institute of Biological Resources (NIBR) under the Ministry of Environment (MOE) and “Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ015247)” of Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon, Republic of Korea

    Sooyeon Park & Jung-Hoon Yoon

  2. Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), 181 Ipsin-gil, Jeongeup, Republic of Korea

    Jung-Sook Lee

  3. Department of Microbiology, Chung-Ang University College of Medicine, 84 Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea

    Wonyong Kim & Jung-Hoon Yoon

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  1. Sooyeon Park
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Correspondence to Jung-Hoon Yoon.

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Park, S., Lee, JS., Kim, W. et al. Mesonia aestuariivivens sp. nov., isolated from a tidal flat. Arch Microbiol 204, 550 (2022). https://doi.org/10.1007/s00203-022-03146-8

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