Abstract
Natural products with antimicrobial activity and their association with synthetic antimicrobials are a sustainable option in fish farming. The objective of this study was to determine antimicrobial activity, antibiofilm potential and synergism of five essential oils (EOs) with florfenicol against motile Aeromonas isolated from Amazonian Colossoma macropomum. As their major constituent, the EOs of the species of Aloysia triphylla, Croton cajucara (red and white morphotype), Cymbopongo citratus and Lippia gracilis present β-pinene (22.1%), germacrene D (11.5%), linalool (23%), geranial (45.7%) and carvacrol (42.2%), respectively. The EOs of L. gracilis and C. citratus showed the best antimicrobial activities against the Aeromonas strains (5 mg mL−1). All EOs interfered with biofilm formation and consolidated biofilm. The EOs of A. triphylla, C. citratus and L. gracilis showed a synergistic effect with florfenicol, reducing the amount of the chemical into the water systems while treatment.
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Data availability statement
The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
We thank Dr. Silvia Gallani at Nilton Lins University for providing the strain of Aeromonas jandaei for the study and Dr. Marcelo Róseo de Oliveira at Embrapa Amazônia Ocidental for assistance in the extraction of essential oils. The authors also thank CNPq (National Council for Scientific and technological development, Brazil) for the productivity research grant awarded to E.C. Chagas (#315771/2020-8). To FAPEAM PAPAC 2020/2021 (Foundation for Research Support in the State of Amazon, Program for the Publication of Scientific Articles and funding for their translation, Resolution 06/2020).
Funding
This research was funded by Embrapa (Brazilian Agricultural Research Corporation—MP2 02.13.09.001.00.00), CNPq (National Council for Scientific and technological development, Brazil) (#315,771/2020-8), FAPEAM PAPAC 2020/2021 (Foundation for Research Support in the State of Amazon, Program for the Publication of Scientific Articles and funding for their translation, Resolution 06/2020).
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ECC and FdAS contributed to the study conception and design. Material preparation, data collection and analysis were performed by all authors. The first draft of the manuscript was written by AMSdS and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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da Silva, A.M.S., Chagas, E.C., Chaves, F.C.M. et al. Prospecting of essential oils in combination with florfenicol against motile Aeromonas isolated from tambaqui (Colossoma macropomum). Arch Microbiol 204, 392 (2022). https://doi.org/10.1007/s00203-022-03015-4
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DOI: https://doi.org/10.1007/s00203-022-03015-4