Abstract
The diversity of endophytic bacteria in the progeny is related to the parental lines. In this study, the traditional separation method was used to study the dominant endophytic bacteria of the shared paternal line and its pollen, different maternal lines and their F1 progeny. And the results showed that the dominant endophytic bacteria in maize seeds and the pollen were Bacillus and Pantoea. The Bacillus diversity of the progeny JMC121 and JN728 were the same as both the paternal line and the maternal line, including Bacillus subtilis, Bacillus velezensis, Bacillus mojavensis, and Bacillus licheniformis. The Bacillus subtilis and Bacillus velezensis in JN828 were the same as both the paternal line and the maternal line, while Bacillus licheniformis was only the same as the paternal line. Through the RAPD molecular typing, there was the same strain of Bacillus mojavensis existed in the paternal line J2416, the pollen and the progeny JN728; this meant that the paternal line passed its dominant endophytic bacteria to the progeny through pollen in vertical transmission. This study showed that the dominant endophytic bacteria in maize seeds and the pollen were Bacillus, and the diversity of F1 progeny was related to both the paternal line and the maternal line.
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The research was supported by the Fundamental Research Funds for the Central Universities (No. FRF-TP-20-044A2; FRF-BR-19-003B; FRF-MP-20–39).
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Wu QY and Wang ZS designed and participated in all experimental procedures, performed data analysis, and drafted the manuscript. Zhang RY and Xu TJ participated in the plant samples cultivation, samples collection and preparation. Zhao JR and Liu Y supervised the study and critically revised the manuscript. All authors read and approved the final manuscript.
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Wu, X., Wang, Z., Zhang, R. et al. Diversity of endophytic bacteria in hybrid maize seeds and Bacillus mojavensis J2416-7 may be capable of vertical transmission. Arch Microbiol 204, 213 (2022). https://doi.org/10.1007/s00203-022-02824-x
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DOI: https://doi.org/10.1007/s00203-022-02824-x