Abstract
A novel extremely halophilic archaeon, designated WN019T, was isolated from the natural saline-alkali wetland soil of Binhai new district, Tianjin, China. Cells of WN019T were aerobic, motile, and pleomorphic rod-shaped, 0.5–0.8 µm in width and 2.0–2.5 µm in length, and the growth occurred optimally at 33–37 °C, pH 7.5–8.0, and in the presence of 15.0–20.0% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequence comparison showed that the isolate belonged to the genus Halorubrum and exhibited moderate sequence similarity of 97.8% to Halorubrum saccharovorum JCM 8865T. The major respiratory quinones of strain WN019T were MK-8 and MK-8 (H2), and the major polar lipids were glycolipid (GL), phospholipid (PL), phosphatidylglycerol-sulphate (PGS), phosphatidylglycerol (PG) and phosphatidylglycerol-phosphate-methyl ester (Me-PGP). The DNA G + C content of the strain was 67.4 mol%. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) value based on whole genome sequences of strain WN019T and Halorubrum saccharovorum JCM 8865T were 87.5% and 35.4%, respectively. Phenotypic, chemotaxonomic, phylogenetic, and genomic analyses suggested that strain WN019T represents a novel species of the genus Halorubrum, for which the name Halorubrum salipaludis sp. nov. is proposed. The type strain is WN019T (= KCTC 4269T = ACCC 19977T).
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Acknowledgements
This work was supported by Science and Technology Partnership Program, Ministry of Science and Technology of China (KY202002003), National Natural Science Foundation of China (NSFC No. 31670113). We would like to thank Prof. Aharon Oren for very valuable help in naming the organism.
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Gong, Q., Zhao, P., Miao, S. et al. Halorubrum salipaludis sp. nov., isolated from the saline–alkaline soil. Arch Microbiol 204, 103 (2022). https://doi.org/10.1007/s00203-021-02729-1
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DOI: https://doi.org/10.1007/s00203-021-02729-1