Abstract
Sequences targeted at the V3 and V4 16S rRNA hypervariable regions of a streptococcal strain (P1L01T) isolated from vaginal swabs of a pregnant woman with diabetes were 100% similar to those of Streptococcus anginosus subsp. whileyi. However, phylogenetic analysis based on 16S rRNA full-gene sequencing (1562 bp) revealed highest sequence similarity to Streptococcus periodonticum (98.7%), followed by Streptococcus anginosus subsp. whileyi (98.7%), and Streptococcus anginosus subsp. anginosus (98.4%). Phylogenies of housekeeping genes rpoB and groEL were compared to improve classification, and the results showed a clear separation between strain P1L01T and closely related Streptococcus type strains. The complete genome of strain P1L01T consisted of 2,108,769 bp with a G + C content of 38.5 mol%. Average nucleotide identity values, based on genome sequencing, between strain P1L01T and Streptococcus periodonticum KCOM 2412T, Streptococcus anginosus subsp. whileyi CCUG 39159T, and Streptococcus anginosus subsp. anginosus NCTC 10713T were 95.5%, 94.3%, and 95.3%, respectively. The highest in silico DNA–DNA hybridization value with respect to the closest species was 66.2%, i.e., below the species cutoff of 70% hybridization. The main cellular fatty acids of strain P1L01T were 16:0, 18:1ω7c, and 14:0. On the basis of phylogenetic, genotypic and phenotypic data, we propose to classify this isolate as representative of a novel species of the genus Streptococcus, Streptococcus vaginalis sp. nov., in reference to its isolation from vaginal swabs, with strain P1L01T (= NBRC 114754T = BCRC 81289T) as the type strain.
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Acknowledgements
We are grateful to Jung-Erh Yang and Hui-Jin Liao for their excellent technical assistance.
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This study was supported by Chang Gung Medical Foundation (CMRPG3H0711, CMRPG3J0771, CMRPG3J1731 and CMRPVVJ0031/2).
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All participants signed an informed consent to authorize the use of samples for research purposes. This study was approved by the Ethics Committee of Research of Chang Gung Memorial Hospital (IRB NO. 201701371A3).
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Communicated by Erko Stackebrandt.
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The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA is LC553197. The Whole Genome Shotgun project has been deposited at DDBJ/ENA/GenBank under the accession JAFINR000000000. The version described in this paper is version JAFINR010000000. Strain P1L01T (Streptococcus vaginalis sp. nov.) has been deposited in the BCRC (Bioresource Collection and Research Centre) and NBRC (Biological Resource Center, NITE) strain collections under numbers 81289 and 114,754, respectively.
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Chao, AS., Lin, CY., Chao, A. et al. Streptococcus vaginalis sp. nov., a novel bacterial species isolated from vaginal swabs of a pregnant woman with diabetes. Arch Microbiol 203, 5475–5482 (2021). https://doi.org/10.1007/s00203-021-02532-y
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DOI: https://doi.org/10.1007/s00203-021-02532-y