Skip to main content

Advertisement

SpringerLink
Log in

The study of stress conditions on growth and proteome of Raoultella planticola: a new emerging pathogen

  • Original Paper
  • Published:
Archives of Microbiology Aims and scope Submit manuscript

Abstract

All bacteria can survive and adapt to different stresses, such as fluctuations in temperature, pH oxidative, and osmotic pressure occurring in their surrounding environments. This study aims to evaluate the effects of a variety of stress conditions on the growth, and proteome of Raoultella planticola PTCC 1598. R. planticola cells were exposed to different values of temperatures, sodium chloride, pH, and hydrogen peroxide stresses. Among the stress conditions, oxidative stress, upon exposure to hydrogen peroxide (H2O2) at 4000 ppm concentration was selected for proteomics analysis in detail. Approximately, 1400 spots were identified in two-dimensional gel electrophoresis (2-DE). Among the identified spots, 85 spots were repeatable using 2D-Platinum software and eye confirmation and, nine protein spots were differentially expressed. Among nine proteins, six proteins identified successfully with an MASCOT score greater than 40 (p < 0.05) were 2,3-dihydroxybenzoate-2,3-dehydrogenase (oxidoreductase family), hypothetical protein G787-04832, periplasmic d-galactose-binding protein, uridine phosphorylase (glycosyltransferases), a single peptide match to cysteine-binding periplasmic protein, and NADP(H) nitroreductase. All identified proteins showed decreased level expression. Based on the obtained results, we concluded that hydrogen peroxide as an antiseptic compound could affect cell growth and proteomics of R. planticola. Therefore, we recommend using an antiseptic solution containing H2O2 to prevent the spread of R. planticola as a new emerging pathogen.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Baez A, Shiloach J (2013) Escherichia coli avoids high dissolved oxygen stress by activation of SoxRS and manganese-superoxide dismutase. Microb Cell Fact 12(1):23

    Article  CAS  Google Scholar 

  • Bagley ST, Seidler RJ, Brenner DJ (1981) Klebsiella planticola sp. Nov.: a new species of Enterobacteriaceae found primarily in nonclinical environments. Curr Microbiol 6(2):105–109

    Article  CAS  Google Scholar 

  • Bartholomäus A, Fedyunin I, Feist P, Sin C, Zhang G, Valleriani A, Ignatova Z (2016) Bacteria differently regulate mRNA abundance to specifically respond to various stresses. Philos Trans A Math Phys Eng Sci. https://doi.org/10.1098/rsta.2015.0069

    Article  PubMed  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72(1–2):248–254

    Article  CAS  Google Scholar 

  • Cho YJ, Jung EJ, Seong JS, Woo YM, Jeong BJ, Kang YM, Lee E (2016) A case of pneumonia caused by Raoultella planticola. Tuberc Respir Dis (Seoul) 79(1):42–45

    Article  Google Scholar 

  • Daware V, Kesavan S, Patil R, Natu A, Kumar A, Kulkarni M, Gade W (2012) Effects of arsenite stress on growth and proteome of Klebsiella pneumoniae. J Biotechnol 158(1–2):8–16

    Article  CAS  Google Scholar 

  • Fager C, Yurteri-Kaplan L (2019) Urinary tract infection with rare pathogen Raoultella planticola: a post-operative case and review. Urol Case Rep 22:76–79

    Article  Google Scholar 

  • Farahtaj F, Zandi F, Khalaj V, Biglari P, Fayaz A, Vaziri B (2013) Proteomics analysis of human brain tissue infected by street rabies virus. Mol Biol Rep 40(11):6443–6450

    Article  CAS  Google Scholar 

  • Garavito MF, Narváez-Ortiz HY, Zimmermann BH (2015) Pyrimidine metabolism: dynamic and versatile pathways in pathogens and cellular development. J Transl Genet Genom 42(5):195–205

    Article  CAS  Google Scholar 

  • Guo MS, Gross CA (2014) Stress-induced remodeling of the bacterial proteome. Curr Biol 24(10):R424–R434

    Article  CAS  Google Scholar 

  • Hayyan M, Hashim MA, AlNashef IM (2016) Superoxide ion: generation and chemical implications. Chem Rev 116(5):3029–3085

    Article  CAS  Google Scholar 

  • Holden VI, Breen P, Houle S, Dozois CM, Bachman MA (2016) Klebsiella pneumoniae siderophores induce inflammation, bacterial dissemination, and HIF-1α stabilization during pneumonia. MBio 7(5):e01397-e1416

    Article  CAS  Google Scholar 

  • Khalil S, Pawelek PD (2011) Enzymatic adenylation of 2,3-dihydroxybenzoate is enhanced by a protein−protein interaction between Escherichia coli 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (EntA) and 2,3-dihydroxybenzoate-AMP ligase (EntE). Biochemistry 50(4):533–545

    Article  CAS  Google Scholar 

  • Kim H-Y, Song H-G (2005) Purification and characterization of NAD (P) H-dependent nitroreductase I from Klebsiella sp. C1 and enzymatic transformation of 2,4,6-trinitrotoluene. Appl Microbiol Biotechnol 68(6):766–773

    Article  Google Scholar 

  • Lam PW, Salit IE (2014) Raoultella planticola bacteremia following consumption of seafood. Can J Infect Dis Med Microbiol 25(4):e83–e84

    Article  Google Scholar 

  • Liu X, Sun X, Wu Y, Xie C, Zhang W, Wang D et al (2013) Oxidation-sensing regulator AbfR regulates oxidative stress responses, bacterial aggregation, and biofilm formation in Staphylococcus epidermidis. J Biol Chem 288(6):3739–3752

    Article  CAS  Google Scholar 

  • McDonnell G (2014) The Use of Hydrogen Peroxide for Disinfection and Sterilization ApplicationsPeroxides.https://doi.org/10.1002/9780470682531.pat0885

  • Montero IG, Dolata KM, Schlüter R, Malherbe G, Sievers S, Zühlke D et al (2019) Comparative proteome analysis in an Escherichia coli CyDisCo strain identifies stress responses related to protein production, oxidative stress and accumulation of misfolded protein. Microb Cell Fact 18(1):1–15

    Article  Google Scholar 

  • Nandakumar M, Shen J, Raman B, Marten MR (2003) Solubilization of trichloroacetic acid (TCA) precipitated microbial proteins via NaOH for two-dimensional electrophoresis. J Proteome Res 2(1):89–93

    Article  CAS  Google Scholar 

  • Nathan C, Ding A (2010) SnapShot: reactive oxygen intermediates (ROI). Cell 140(6):951

    Article  Google Scholar 

  • Niedergethmann M, Alves F, Neff J, Heidrich B, Aramin N, Li L et al (2007) Gene expression profiling of liver metastases and tumour invasion in pancreatic cancer using an orthotopic SCID mouse model. Br J Cancer 97(10):1432–1440

    Article  CAS  Google Scholar 

  • O'Connell K, Kelly J , NiRiain U (2010) A rare case of soft-tissue infection caused by Raoultella planticola. J Med Case Rep 2010

  • Ohtsu I, Kawano Y, Suzuki M, Morigasaki S, Saiki K, Yamazaki S et al (2015) Uptake of l-cystine via an ABC transporter contributes defense of oxidative stress in the l-cystine export-dependent manner in Escherichia coli. PLoS ONE 10(4):e0120619

    Article  Google Scholar 

  • Olson D, Asare K, Lyons M, Hofinger D (2013) A novel case of Raoultella planticola urinary tract infection. Infection 41(1):259–261

    Article  Google Scholar 

  • Pan S, Rodriguez D, Thirumavalavan N, Gross MS, Eid JF, Mulcahy J, Munarriz R (2019) The use of antiseptic solutions in the prevention and management of penile prosthesis infections: a review of the cytotoxic and microbiological effects of common irrigation solutions. J Sex Med 16(6):781–790

    Article  Google Scholar 

  • Siauciunaite R, Foulkes NS, Calabrò V, Vallone D (2019) Evolution shapes the gene expression response to oxidative stress. Int J Mol Sci 20(12):3040. https://doi.org/10.3390/ijms20123040

    Article  CAS  PubMed Central  Google Scholar 

  • Tang P, Liu JK, Chou SM, Hor LI, Chen WJ, Chen SC (2008) A proteomic analysis of Klebsiella oxytoca after exposure to succinonitrile. Process Biochem 43(7):753–757

    Article  CAS  Google Scholar 

  • Tantasuttikul A, Mahakarnchanakul W (2019) Growth parameters and sanitizer resistance of Raoultella ornithinolytica and Raoultella terrigena isolated from seafood processing plant. Cogent Food Agric 5(1):1569830. https://doi.org/10.1080/23311932.2019.1569830

    Article  CAS  Google Scholar 

  • Van Meurs S, Gawlitta D, Heemstra K, Poolman R, Vogely H, Kruyt M (2014) Selection of an optimal antiseptic solution for intraoperative irrigation: an in vitro study. JBJS 96(4):285–291

    Article  Google Scholar 

  • Westerveld D, Hussain J, Aljaafareh A, Ataya A (2017) A rare case of Raoultella planticola pneumonia: an emerging pathogen. Respir Med Case Rep 21:69–70

    PubMed  PubMed Central  Google Scholar 

  • Young I, Gibson F (1969) Regulation of the enzymes involved in the biosynthesis of 2,3-dihydroxybenzoic acid in Aerobacter aerogenes and Escherichia coli. Biochim Biophys Acta Gen Subj 177(3):401–411

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The laboratory facilities of microbial experiments were provided by Lahijan Branch, Islamic Azad University, Lahijan, Iran. The authors are grateful to Dr. Behrouz Vaziri and Mrs. Bahareh Azarian at Protein Chemistry Unit, Biotechnology Research Center, Pasteur Institute of Iran and ProteomicsTechnology Facility, Department of Biology, the University of York UK for their helpful assistance in proteomics analysis.

Funding

This research did not receive any specific Grant from funding agencies in the public, commercial, or not-for-profit sectors and the authors financially supported this work.

Author information

Authors and Affiliations

  1. Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University, P.O. Box 1616, Lahijan, Iran

    Zeynab Hajian, Mohammad Faezi Ghasemi & Fatemeh (Elham) Alikhani

Authors
  1. Zeynab Hajian
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mohammad Faezi Ghasemi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Fatemeh (Elham) Alikhani
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

This study was performed based on a Master of Science (MSc) thesis addressed to Zeynab Hajian. ZH, MFG, and FEA contributed almost equally to this study. MFG¸ designed the study, ZH and FEA did the experiments and performed research; MFG analyzed all data and wrote the paper.

Corresponding author

Correspondence to Mohammad Faezi Ghasemi.

Ethics declarations

Conflict of interest

The author declares that they have no conflict of interest.

Additional information

Communicated by Erko Stackebrandt.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Hajian, Z., Ghasemi, M.F. & Alikhani, F.(. The study of stress conditions on growth and proteome of Raoultella planticola: a new emerging pathogen. Arch Microbiol 203, 3269–3278 (2021). https://doi.org/10.1007/s00203-021-02312-8

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00203-021-02312-8

Keywords

Search

Navigation