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Diversity of the type I-U CRISPR-Cas system in Bifidobacterium

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Abstract

The CRISPR-Cas system is widely distributed in prokaryotes and plays an important role in the adaptive immunity of bacteria and archaea. Bifidobacterium is an important component of the intestinal flora of humans and animals, and some species of this bacterium can be employed as food additives. However, the Bifidobacterium CRISPR-Cas system has not been fully elucidated to date. In this study, the genomes of 110 strains of Bifidobacterium were employed to research the diversity of the type I-U system. The 110 strains were divided into five groups according to the genes adjacent to the CRISPR locus, including group A, B, C, D and E. Strains in the intergroup had unique species classifications and MLST types. An evolutionary tree was constructed based on the conserved cas4/cas1 fusion gene. The results showed that group A had a different evolutionary branch compared with the other groups and had a relatively low spacer number. Notably, group B, C and E had exhibited ABC transporter regulators in the genes adjacent to the CRISPR locus. ABC transporters play important roles in the exocytosis of many antibiotics and are involved in horizontal gene transfer. This mechanism may have promoted the evolution of Bifidobacterium and the horizontal gene transfer of the type I-U system, which may have promoted the generation of system diversity. In summary, our results help to elucidate the role of the type I-U system in the evolution of Bifidobacterium.

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Funding

The work was funded by the National Science and Technology Specific Projects (2018ZX10301407), the Key Scientific Research Project of Colleges and Universities in Henan Province (Grant No. 20A330004) and the National Natural Science Foundation of China (Grant No. 81573205).

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Authors and Affiliations

  1. Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou, People’s Republic of China

    Liuyang Ou, Jinzhao Long, Yanli Teng, Haiyan Yang, Yuanlin Xi, Guangcai Duan & Shuaiyin Chen

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  1. Liuyang Ou
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  2. Jinzhao Long
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Contributions

LO, GD and SC designed the study, and LO, JL and YT analyzed the data and wrote the paper. HY and YX collected some data. All authors read and approved the final manuscript.

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Correspondence to Guangcai Duan or Shuaiyin Chen.

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Communicated by Erko Stackebrandt.

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Ou, L., Long, J., Teng, Y. et al. Diversity of the type I-U CRISPR-Cas system in Bifidobacterium. Arch Microbiol 203, 3235–3243 (2021). https://doi.org/10.1007/s00203-021-02310-w

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