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Bombilactobacillus apium sp. nov., isolated from the gut of honeybee (Apis cerana)

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Abstract

A novel Gram-reaction positive-, catalase and oxidase negative-, rod-shaped, facultatively anaerobic bacterial strain, DCY120T, was isolated from the gut of honeybee (Apis cerana) in Gyeonggi-do, South Korea. Strain DCY120T belongs to the genus Bombilactobacillus and is moderately related to Bombilactobacillus mellis Hon2T (94.1% similarity), Bombilactobacillus bombi BTLCH M1/2T (93.8%), and Bombilactobacillus mellifer Bin4NT (93.5%) based on 16S rRNA gene sequence analysis. The genome of strain DCY120T was sequenced and the average nucleotide identity (ANI) between strain DCY120T and the related Bombilactobacillus type strains were below the threshold value (95–96%) for species delineation. The major fatty acids were C16:0, C18:1 ω9c, Summed C19:1 ω6c/C19:0 cyclo ω10c/C19:0 ω6 and Summed C18:1 ω7c/C18:1 ω6c. The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), one glycolipid (GL), and one unidentified aminophospholipid (APL). The amino acids in peptidoglycan of strain DCY120T were lysine, alanine, glutamic acid, and aspartic acid. In conclusion, the description of phenotypic and genotypic properties support strain DCY120T as a novel species within the genus Bombilactobacillus, for which the name Bombilactobacillus apium sp. nov. is proposed. The type strain is DCY120T (= KCTC 43194T = JCM 34006T).

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Acknowledgments

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2019R1A6A3A01094826). The NCBI GenBank accession numbers for the 16S rRNA gene sequence and the draft genome of strain DCY120T are MN709051 and JABZEC000000000, respectively.

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Author notes

  1. Jong-Pyo Kang and Yue Huo contributed equally to this work.

Authors and Affiliations

  1. Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Seocheon-dong, Giheung-gu, Yongin-si, Gyeonggi-do, 17104, Republic of Korea

    Jong-Pyo Kang, Yue Huo, Deok-Chun Yang & Se-Chan Kang

  2. Department of Oriental Medicinal Biotechnology, Kyung Hee University, Seocheon-dong, Giheung-gu, Yongin-si, Gyeonggi-do, 17104, Republic of Korea

    Van-An Hoang, Dong-Uk Yang & Deok-Chun Yang

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  1. Jong-Pyo Kang
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  2. Yue Huo
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  3. Van-An Hoang
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  4. Dong-Uk Yang
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  5. Deok-Chun Yang
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Correspondence to Deok-Chun Yang or Se-Chan Kang.

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Communicated by Erko Stackebrandt.

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Supplementary Information

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203_2021_2249_MOESM1_ESM.docx

Supplementary file1 (DOCX 2475 KB). Fig. S1. Neighbor-joining phylogenetic tree based on 16S rRNA gene sequences (1457 bp), showing the taxonomic position of strain DCY120T in the genus Bombilactobacillus. Bootstrap values > 50% based on 1000 replications are shown at branching points. Paenibacillus larvae DSM 7030T (AY530294) was used as an out group. Bar, 0.02 substitutions per nucleotide position. Fig. S2. Two-dimensional TLC of the total lipids of strain DCY120T (A) and Bombilactobacillus mellis DSM 26255T (B) stained with 5% ethanolic molybdophosphoric acid. DPG, diphosphatidylglycerol; PG, phosphatidylglycerol; GL 1-2, glycolipid; APL, aminophospholipid; PGL, phosphoglycolipid; L, unidentified polar lipids. Fig. S3. The one-dimensional thin layer chromatograms of cell-wall peptidoglycan amino acids of strain DCY120T. Table S1. Negative characteristics of strain DCY120T in API ZYM and API 50 CH tests

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Kang, JP., Huo, Y., Hoang, VA. et al. Bombilactobacillus apium sp. nov., isolated from the gut of honeybee (Apis cerana). Arch Microbiol 203, 2193–2198 (2021). https://doi.org/10.1007/s00203-021-02249-y

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  • DOI: https://doi.org/10.1007/s00203-021-02249-y

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