Skip to main content
SpringerLink
Log in

Chelativorans xinjiangense sp. nov., a novel bacterial species isolated from soil in Xinjiang, China

  • Original Paper
  • Published:
Archives of Microbiology Aims and scope Submit manuscript

Abstract

A novel Gram-strain-negative, beige-pigmented, aerobic, rod-shaped, non-flagellated and non-gliding bacterium, designated strain lm93T, was isolated from rhizosphere soil of Alhagi sparsifolia obtained from Alar city, located in Xinjiang province, China. Growth optimally occurred at 30 °C, pH 6.5–7.5, and 0–2% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain lm93T belonged to the genus Chelativorans, with highest sequence similarity to Chelativorans multitrophicus DSM 9103T (96.9%). Genome sequencing revealed a genome size of 5 689 708 bp and a G + C content of 64.3 mol%. The ANI, POCP and the dDDH between strain lm93T and C. multitrophicus DSM 9103T were 76.4%, 54.8% and 0.8%, respectively. The prediction result of secondary metabolites based on genome showed that the strain lm93T contained one cluster of bacteriocin, one cluster of terpene production, two clusters of ectoine production, one cluster of non-ribosomal peptide synthetase, one cluster of type I polyketide synthases, three clusters of homoserine lactone production, one cluster of N-acetylglutaminylglutamine amide production and one cluster of phosphonate production. The major respiratory quinone was Q-10. The major fatty acids were C19:0 cyclo ω8c, iso-C17:0 and summed feature 8 (C18:1 ω6c and/or C18:1 ω7c) and its polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, two unidentified aminophospholipids, aminoglycolipid, three unknown lipids and diphosphatidylglycerol. On the basis of these data, strain lm93T is considered to represent a novel species of the genus Chelativorans, for which the name Chelativorans xinjiangense sp. nov. is proposed. The type strain is lm93T (= KCTC 72857T = CCTCC AB2019376T).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

Abbreviations

ANI:

Average nucleotide identity

dDDH:

Digital DNA–DNA hybridization

HPLC:

High performance liquid chromatography

POCP:

Percentage of conserved proteins

MIDI:

Microbial identification system

KCTC:

Korean collection for type cultures

CCTCC:

China center for type culture collection

TLC:

Thin layer chromatography

References

  • Blin K, Shaw S, Steinke K, Villebro R, Ziemert N, Lee SY, Medema MH, Weber T (2019) antiSMASH 5.0: Updates to the secondary metabolite genome mining pipeline. Nucleic Acids Res 47:W81–W87

    Article  CAS  Google Scholar 

  • Bowman JP (2000) Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. Int J Syst Evol Microbiol 50:1861–1868

    Article  CAS  Google Scholar 

  • Cerny G (1978) Studies on the aminopeptidase test for the distinction of gram-negative from gram-positive bacteria. Eur J Appl Microbiol 5:113–122

    Article  CAS  Google Scholar 

  • Collins MD, Pirouz T, Goodfellow M, Minnikin DE (1977) Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230

    Article  CAS  Google Scholar 

  • Doronina NV, Kaparullina EN, Trotsenko YA, Nörtemann B, Bucheliwitschel M, Weilenmann HU, Egli T (2010) Chelativorans multitrophicus gen. nov., sp. nov. and Chelativorans oligotrophis sp. nov., aerobic EDTA-degrading bacteria. Int J Syst Evol Microbiol 60:1044–1051

    Article  CAS  Google Scholar 

  • Fang BZ, Xie YG, Zhou XK, Zhang XT, Liu L, Jiao JY, Xiao M, Li WJ (2020) Lysobacter prati sp. nov., isolated from a plateau meadow sample. Anton Leeuw J Microbiol 113:763–772

    Article  CAS  Google Scholar 

  • Institute C (2007) Performance standards for antimicrobial susceptibility testing. Twenty-second informational supplement, vol 31. Cornell University, New York, pp 76–79

    Google Scholar 

  • Kämpfer P, Arun AB, Busse HJ, Zhang ZL, Glaeser SP (2015) Chelativorans intermedius sp. nov. and proposal to reclassify Thermovum composti as Chelativorans composti comb. nov. Int J Syst Evol Microbiol 65:1646–1652

    Article  Google Scholar 

  • Kroppenstedt RM (1982) Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5:2359–2367

    Article  CAS  Google Scholar 

  • Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874

    Article  CAS  Google Scholar 

  • Liu YL, Meng D, Li R-R, Gu P-F, Li Q (2019) Rhodoligotrophos defluvii sp. nov., isolated from activated sludge. Int J Syst Evol Microbiol 69:3830–3836

    Article  CAS  Google Scholar 

  • Marchesi JR, Sato T, Weightman AJ, Martin TA, Fry JC, Hiom SJ, Dymock D, Wade WG (1998) Design and evaluation of useful bacterium-specific pcr primers that amplify genes coding for bacterial 16s rrna. Appl Environ Microbiol 64:795–799

    Article  CAS  Google Scholar 

  • Minnikin DE, Collins MD, Goodfellow M (1979) Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47:87–95

    Article  CAS  Google Scholar 

  • Minnikin D, O’Donnell A, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Appl Bacteriol 2:233–241

    CAS  Google Scholar 

  • Mitchell AL, Attwood TK, Babbitt PC (2019) InterPro in 2019: improving coverage, classification and access to protein sequence annotations. Nucleic Acids Res 47:D351–D360

    Article  CAS  Google Scholar 

  • Parte AC (2013) LPSN—list of prokaryotic names with standing in nomenclature. Nucleic Acids Res 42:D613–D616

    Article  Google Scholar 

  • Qin QL, Xie BB, Zhang XY, Chen XL, Zhou BC, Zhou J, Oren A, Zhang YZ (2014) A proposed genus boundary for the prokaryotes based on genomic insights. J Bacteriol 196:2210–2215

    Article  Google Scholar 

  • Richter M, Rosselló-Móra R (2009) Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 106:19126–19131

    Article  CAS  Google Scholar 

  • Richter M, Rosselló-Móra R, Glökner FO, Peplies J (2015) JSpeciesWS: a web server for prokaryotic species circumscription based on pairwise genome comparison. Bioinformatics 32:929–931

    Article  Google Scholar 

  • Sashidhar B, Podile AR (2010) Mineral phosphate solubilization by rhizosphere bacteria and scope for manipulation of the direct oxidation pathway involving glucose dehydrogenase. J Appl Microbiol 109:1–12

    CAS  PubMed  Google Scholar 

  • Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Tech Note 101:1–6

    Google Scholar 

  • Yoon SH, Ha SM, Kwon S, Lim J, Kim Y, Seo H, Chun J (2017) Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 67:1613–1617

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by the National Natural Science Foundation of China (31870105, 31741007), and the Training Foundation of Shandong Natural Science Foundation (ZR2019PEE030).

Author information

Authors and Affiliations

  1. School of Biological Science and Technology, University of Jinan, Jinan, 250022, People’s Republic of China

    Dong Meng, Yu-Ling Liu, Peng-Fei Gu, Xiang-Yu Fan, Zhao-Song Huang, Yan Ji & Qiang Li

  2. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, People’s Republic of China

    Wei-Min Li

  3. Key Laboratory for Northern Urban, Agriculture of Ministry of Agriculture and Rural Affairs, Beijing University of Agriculture, Beijing, 102206, People’s Republic of China

    Wei-Min Li

  4. Marine College, Shandong University, Weihai, 264209, People’s Republic of China

    Zong-Jun Du

Authors
  1. Dong Meng
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yu-Ling Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Peng-Fei Gu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xiang-Yu Fan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Zhao-Song Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yan Ji
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Wei-Min Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Zong-Jun Du
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Qiang Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Author contributions MD, LWM and LQ designed research and project outline. JY, LYL, FXY and HZS performed isolation, deposition, and identification. MD, GPF, DZJ, LWM and LQ drafted the manuscript. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Wei-Min Li or Qiang Li.

Ethics declarations

Conflict of interests

The authors declare that they have no conflicts of interest.

Ethical statements

This article does not contain any studies with animals performed by any of the authors.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Additional information

Communicated by Erko stackebrandt.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

The GenBank accession number for the 16S rRNA gene sequence of Chelativorans xinjiangense lm93T is MN372073. The whole genome shotgun project of strain lm93T has been deposited at DDBJ/ENA/GenBank under the accession number WQNH00000000. The version described in this paper is version WQNH00000000.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 1345 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Meng, D., Liu, YL., Gu, PF. et al. Chelativorans xinjiangense sp. nov., a novel bacterial species isolated from soil in Xinjiang, China. Arch Microbiol 203, 693–699 (2021). https://doi.org/10.1007/s00203-020-02064-x

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00203-020-02064-x

Keywords

Search

Navigation