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Inhibition of biofilm formation of Pseudomonas aeruginosa by caffeine: a potential approach for sustainable management of biofilm

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Abstract

Pseudomonas aeruginosa is a potent biofilm forming organism causing several diseases on host involving biofilm. Several natural and synthetic molecules have been explored towards inhibiting the biofilm formation of Pseudomonas aeruginosa. In the current report, the role of a natural molecule namely caffeine was examined against the biofilm forming ability of P. aeruginosa. We have observed that caffeine shows substantial antimicrobial activity against P. aeruginosa wherein the minimum inhibitory concentration (MIC) of caffeine was found to be 200 μg/mL. The antibiofilm activity of caffeine was determined by performing a series of experiments using its sub-MIC concentrations (40 and 80 μg/mL). The results revealed that caffeine can significantly inhibit the biofilm development of P. aeruginosa. Caffeine has been found to interfere with the quorum sensing of P. aeruginosa by targeting the swarming motility. Molecular docking analysis further indicated that caffeine can interact with the quorum sensing proteins namely LasR and LasI. Thus, the result indicated that caffeine could inhibit the formation of biofilm by interfering with the quorum sensing of the organism. Apart from biofilm inhibition, caffeine has also been found to reduce the secretion of virulence factors from Pseudomonas aeruginosa. Taken together, the results revealed that in addition to biofilm inhibition, caffeine can also decrease the spreading of virulence factors from Pseudomonas aeruginosa.

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Acknowledgements

PC, DD, PP, SD, DB, SS, SB, RS performed the experiments, analyzed the results and wrote the manuscript. AS, AS designed the experiments, analyzed the results and wrote the manuscript. PT conceived the idea, designed the experiments, analyzed the results and wrote the manuscript.

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Authors and Affiliations

  1. Department of Biotechnology, The Neotia University, Sarisha, West Bengal, 743368, India

    Poulomi Chakraborty, Payel Paul, Sutirtha Dutta, Debajjyoti Basu, Ranojit Kumar Sarker, Amit Sarkar & Prosun Tribedi

  2. Department of Biotechnology, Guru Nanak Institute of Pharmaceutical Science and Technology, Kolkata, West Bengal, 700014, India

    Debabrata Ghosh Dastidar

  3. Department of Microbiology, Lady Brabourne College, Kolkata, West Bengal, 700017, India

    Senjuti Roy Sharma, Shreosi Basu & Aparna Sen

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  1. Poulomi Chakraborty
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  2. Debabrata Ghosh Dastidar
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  3. Payel Paul
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  4. Sutirtha Dutta
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Correspondence to Prosun Tribedi.

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Communicated by Erko Stackebrandt.

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Chakraborty, P., Dastidar, D.G., Paul, P. et al. Inhibition of biofilm formation of Pseudomonas aeruginosa by caffeine: a potential approach for sustainable management of biofilm. Arch Microbiol 202, 623–635 (2020). https://doi.org/10.1007/s00203-019-01775-0

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  • DOI: https://doi.org/10.1007/s00203-019-01775-0

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