Abstract
The hemicellulolytic enzyme system produced by Cellulosimicrobium cellulans strain F16 was resolved by ultracentrifugation and size exclusion chromatography. The particle size and molecular weight were determined by both dynamic light scattering and negative stain electron microscopy. The results showed that xylanosomes produced by strain F16 were found to have an apparent sedimentation coefficient of 28 S, were diverse in size (18–70 nm), molecular weight (11–78 MDa) and morphology, but resembled in subunit composition (SDS-PAGE and proteomic results). It is proposed that particles of 22 nm may be the basic unit, while 43 nm and 60 nm particles observed may be dimer and trimer of the basic unit, or xylanosomes with smaller size might be degradation products of larger size xylanosomes. Moreover, such xylanosomes are also found to have strong binding affinity toward water-insoluble substrates such as Avicel, birchwood xylan, and corn cob.
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Abbreviations
- DLS:
-
Dynamic light scattering
- SEC:
-
Size exclusion chromatography
- DAXP:
-
7-Xylosyl-10-deacetylpaclitaxel
- TEM:
-
Transmission electron microscopy
- MUX:
-
4-Methylumbelliferyl-U-β-d-xylopyranoside
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Acknowledgements
The authors would like to thank Dr. Hongwei Luan, Xingbao Liu, and Shiyang Li for fermentation and ultrafiltration assistances.
Funding
This work was supported by the National Natural Science Foundation of China (No. 31600641), the National Key Research and Development Program of China (2017YFC1702006), and the Fundamental Research Funds for the Central Universities (No. DUT18RC(4)057).
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Communicated by Yusuf Akhter.
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Dou, TY., Chen, J., Hao, YF. et al. Xylanosomes produced by Cellulosimicrobium cellulans F16 were diverse in size, but resembled in subunit composition. Arch Microbiol 201, 163–170 (2019). https://doi.org/10.1007/s00203-018-1606-z
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DOI: https://doi.org/10.1007/s00203-018-1606-z