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Heterogeneity of Bordetella bronchiseptica adenylate cyclase (cyaA) RTX domain

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Abstract

Bordetella bronchiseptica is a widespread pathogen, with a broad host range, occasionally including humans. Diverse virulence factors (adhesins, toxins) allow its adaptation to its host, but this property of the adenylate cyclase (cyaA) toxin is not well understood. In this study, we analyzed the repeats-in-toxin domain of B. bronchiseptica cyaA with PCR, followed by restriction fragment length analysis. Of ninety-two B. bronchiseptica strains collected from different hosts and geographic regions, 72 (78.3 %) carried cyaA and four RFLP types (A–D) were established using NarI and SalI. However, in 20 strains, cyaA was replaced with a peptide transport protein operon. A phylogenetic tree based on partial nucleotide sequences of cyaA revealed that group 2 contains strains of specifically human origin, whereas subgroup 1a contains all but one of the strains from pigs. The human strains showed many PCR–RFLP and sequence variants, confirming the clonal population structure of B. bronchiseptica.

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Acknowledgments

This work was supported by the Hungarian Scientific Research Fund (OTKA, K83332). We thank Prof. Alistair Lax (Microbiology Department of King’s College, London, UK) and Karen B. Register (National Animal Disease Center, USDA, Ames, IA, USA) for providing strains.

Conflict of interest

The authors have no conflicts of interest to declare.

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Authors and Affiliations

  1. Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária krt. 21, 1143, Budapest, Hungary

    Enikő Wehmann, Bernadett Khayer & Tibor Magyar

Authors
  1. Enikő Wehmann
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  2. Bernadett Khayer
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  3. Tibor Magyar
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Correspondence to Enikő Wehmann.

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Communicated by Erko Stackebrandt.

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Wehmann, E., Khayer, B. & Magyar, T. Heterogeneity of Bordetella bronchiseptica adenylate cyclase (cyaA) RTX domain. Arch Microbiol 197, 105–112 (2015). https://doi.org/10.1007/s00203-014-1068-x

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  • DOI: https://doi.org/10.1007/s00203-014-1068-x

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