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Yeast protein phosphatases Ptp2p and Msg5p are involved in G1–S transition, CLN2 transcription, and vacuole morphogenesis

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Abstract

We previously reported that double disruption of protein phosphatase (PPase) genes PTP2 (phosphotyrosine-specific PPase) and MSG5 (phosphotyrosine and phosphothreonine/serine-PPase) causes Ca2+ sensitive growth, whereas the single disruptions do not. This finding suggests that Ptp2p and Msg5p are involved in Ca2+-induced stress response in a redundant manner. To gain insight into the molecular mechanism causing calcium sensitivity of the ∆ptp2 ∆msg5 double disruptant, we performed fluorescence-activated cell sorting analysis and found a delayed G1 phase. This delayed G1 was consistent with the defect in bud emergence, and reduced CLN2 transcription upon addition of CaCl2. We also found that Slt2p is hyper-phosphorylated in the Δptp2 Δmsg5 double disruptant and that the vacuole of the Δptp2 Δmsg5 double disruptant is fragmented even in the absence of Ca2+. These findings suggest that both Ptp2p and Msg5p are involved in the G1 to S transition and vacuole morphogenesis possibly through their regulation of Slt2 pathway.

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Abbreviations

PPase:

Protein phosphatase

MAPK:

Mitogen-activated protein kinase

Cas :

Calcium sensitive

CWI:

Cell wall integrity

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Acknowledgment

This work was supported by a Grant-in-Aid for Scientific Research B, 2007 to 2009, to S.H. from the Ministry of Education, Science, Sports and Culture of Japan.

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Authors and Affiliations

  1. Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan

    Hermansyah, Minetaka Sugiyama, Yoshinobu Kaneko & Satoshi Harashima

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  1. Hermansyah
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  2. Minetaka Sugiyama
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  3. Yoshinobu Kaneko
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  4. Satoshi Harashima
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Corresponding author

Correspondence to Satoshi Harashima.

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Communicated by Axel Brakhage.

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Hermansyah, Sugiyama, M., Kaneko, Y. et al. Yeast protein phosphatases Ptp2p and Msg5p are involved in G1–S transition, CLN2 transcription, and vacuole morphogenesis. Arch Microbiol 191, 721–733 (2009). https://doi.org/10.1007/s00203-009-0498-3

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  • DOI: https://doi.org/10.1007/s00203-009-0498-3

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