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Diverse bacteria isolated from root nodules of wild Vicia species grown in temperate region of China

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Abstract

In the present study, a total of 154 bacterial strains isolated from nodules of eighteen Vicia species mainly grown in the temperate Chinese provinces were characterized by ARDRA, ITS PCR–RFLP, BOX-PCR, sequencing of 16S rDNA, nodC, nifH, atpD and glnII, and nodulation tests. The results demonstrated that most of the R. leguminosarum strains were effective microsymbionts of the wild Vicia species, while genomic species related to Rhizobium gallicum, Mesorhizobium huakuii, Ensifer meliloti and Bradyrhizobium spp. were symbiotic bacteria occasionally nodulating with Vicia species. In addition, fourteen strains related to Agrobacterium, Phyllobacterium, Ensifer, Shinella and R. tropici, as well as 22 strains of R. leguminosarum might be nodule endophytes without symbiotic genes. Diverse symbiotic gene lineages were found among the test strains and a strong association was found among the symbiotic gene types and genomic species, indicating the absence of lateral gene transfer. These results greatly enlarged the rhizobial spectrum of Vicia species.

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Acknowledgments

We thank Dr. C. T. Gu, Dr. J. H. Li, Dr. T. X. Han and other collaborators for their help and valuable suggestions during the study. This work was financial supported by National Basic Research Program of China (grant 2006CB100206), by National Program for Basic S & T Plantform Construction (grant 2005DKA21201-10), and by National Natural Science Foundational of China (grant 30670001). ETW was supported by projects of SIP 20070538 and SIP 20080322 authorized by IPN, Mexico.

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  1. Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture/College of Biological Sciences, China Agricultural University, 100193, Beijing, China

    Xia Lei, Wen Feng Chen, Xin Hua Sui & Wen Xin Chen

  2. Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340, Mexico D.F., Mexico

    En Tao Wang

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  1. Xia Lei
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Correspondence to Wen Xin Chen.

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Communicated by Ursula Priefer.

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Lei, X., Wang, E.T., Chen, W.F. et al. Diverse bacteria isolated from root nodules of wild Vicia species grown in temperate region of China. Arch Microbiol 190, 657–671 (2008). https://doi.org/10.1007/s00203-008-0418-y

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