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Genomics of the ccoNOQP-encoded cbb 3 oxidase complex in bacteria

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Abstract

Many bacteria adapt to microoxic conditions by synthesizing a particular cytochrome c oxidase (cbb 3) complex with a high affinity for O2, encoded by the ccoNOQP operon. A survey of genome databases indicates that ccoNOQP sequences are widespread in all sub-branches of Proteobacteria but otherwise are found only in bacteria of the CFB group (Cytophaga, Flexibacter, Bacteroides). Our analysis of available genome sequences suggests four major strategies of regulating ccoNOQP expression in response to O2. The most widespread strategy involves direct regulation by the O2-responsive protein Fnr. The second strategy involves an O2-insensitive paralogue of Fnr, FixK, whose expression is regulated by the O2-responding FixLJ two-component system. A third strategy of mixed regulation operates in bacteria carrying both fnr and fixLJ-fixK genes. Another, not yet identified, strategy is likely to operate in the ε-Proteobacteria Helicobacter pylori and Campylobacter jejuni which lack fnr and fixLJ-fixK genes. The FixLJ strategy appears specific for the α-subclass of Proteobacteria but is not restricted to rhizobia in which it was originally discovered.

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Acknowledgements

We are grateful to Dr J.V. Cullimore and members of J. Batut’s team for critical reading of the manuscript. Céline Cosseau was supported by a doctoral fellowship from the French Ministère de l’Enseignement Supérieur et de la Recherche.

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  1. Laboratoire des Interactions Plantes-Micro-organismes, UMR CNRS-INRA 2594/441, BP27, 31326 , Castanet Tolosan cedex, France

    Céline Cosseau & Jacques Batut

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  1. Céline Cosseau
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  2. Jacques Batut
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Correspondence to Jacques Batut.

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Cosseau, C., Batut, J. Genomics of the ccoNOQP-encoded cbb 3 oxidase complex in bacteria. Arch Microbiol 181, 89–96 (2004). https://doi.org/10.1007/s00203-003-0641-5

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  • DOI: https://doi.org/10.1007/s00203-003-0641-5

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