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Expression of the trxA gene for thioredoxin 1 in Rhodobacter sphaeroides during oxidative stress

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Abstract

Expression of the thioredoxin (trxA) gene of Rhodobacter sphaeroides is regulated by oxidative stress at the transcriptional and post-transcriptional levels. All oxidative stress agents tested resulted in a moderate or strong increase of trxA mRNA levels, which was not due to increased mRNA stability. While the kinetics of increased trxA mRNA and of sodB mRNA, encoding superoxide dismutase, were similar after addition of tert-butyl hydroperoxide (t-BOOH) or hydrogen peroxide (H2O2), different kinetics were observed after addition of diamide or paraquat, indicating the involvement of different stress responses in the regulation of these genes. The level of TrxA did not increase to the same extent as trxA mRNA levels. Furthermore, the addition of H2O2 or t-BOOH led to increased turnover of the protein. Apparently, increased txA transcription compensated, at least in part, for the reduced stability of the protein. A strain expressing lower levels of thioredoxin 1 showed decreased resistance to diamide and H2O2 but increased resistance to paraquat and t-BOOH compared to the wild-type. These data implicate the involvement of various systems in the response to different types of oxidative stress and the participation of thioredoxin 1 in the defense against oxidative stress caused by diamide or H2O2.

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Abbreviations

H 2 O 2 :

Hydrogen peroxide

ROS :

Reactive oxygen species

SOD :

Superoxide dismutase

t -BOOH:

tert-Butyl hydroperoxide

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Acknowledgements

Kuanyu Li was supported by a fellowship from DAAD (Deutscher Akademischer Austauschdienst). This work was supported by Deutsche Forschungsgemeinschaft (Kl 563/7-3/7-4/16-1) and by Fonds der Chemischen Industrie.

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Author notes

  1. Cécile Pasternak

    Present address: Laboratoire de Génétique des Bacteries Photosynthetiques, Centre de Génétique Moleculaire–Bat. 26, CNRS, 91198 Gif sur Yvette Cedex, France

Authors and Affiliations

  1. Institut für Mikrobiologie und Molekularbiologie, Heinrich-Buff-Ring 26–32, 35392, Giessen, Germany

    Kuanyu Li, Cécile Pasternak & Gabriele Klug

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  1. Kuanyu Li
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  2. Cécile Pasternak
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  3. Gabriele Klug
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Correspondence to Gabriele Klug.

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Li, K., Pasternak, C. & Klug, G. Expression of the trxA gene for thioredoxin 1 in Rhodobacter sphaeroides during oxidative stress. Arch Microbiol 180, 484–489 (2003). https://doi.org/10.1007/s00203-003-0620-x

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  • DOI: https://doi.org/10.1007/s00203-003-0620-x

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