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In vitro and in vivo assessment of silver-coated polypropylene mesh to prevent infection in a rat model

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Abstract

Introduction and hypothesis

The purpose of this study is to determine the effect of silver coating of polypropylene implants on infection in hernia surgery.

Methods

Silver-coated and non-silver-coated large pore monofilament polypropylene mesh implants were compared with and without infection (four groups). The implants were inserted in the abdominal wall of female Wistar rats. An Escherichia coli strain was inoculated intraoperatively in the two infected groups. The implants were removed, and clinical, bacteriological, and histological analyses were performed at 2, 15, and 30 days postoperatively.

Results

Eighty-four rats were studied. All inoculated rats (n = 21) in the non-silver-coated polypropylene group presented periprosthetic E. coli infection, compared with only five inoculated rats in the silver-coated polypropylene group (p < 0.0001). Erosion was significantly higher in the infected than in the non-infected silver-coated polypropylene groups (p < 0.01). There was no histological difference between the four groups.

Conclusions

Silver-coated implants appear effective against bacterial infection in our rat model, with good histological tolerance but delayed healing.

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Acknowledgments

We are indebted to Mrs. Guiraud, Histology–Embryology–Cytogenetic Laboratory, Montpellier-Nîmes Medical University, Nîmes, France and to Prof. Courtade, Histology–Embryology Laboratory, Rangueil Medical University, Toulouse, France for their help in the histological study. We also thank the Bard Urological Division, Covington, KY, USA for creating and donating the polypropylene and the silver-coated polypropylene implant materials, and for partial support of the study.

Conflicts of interest

This study was partially funded by Bard Urological Division. Renaud de Tayrac is Consultant for Boston Scientific.

Author information

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Paule de Viguier Hospital, 31000, Toulouse, France

    Wassim Badiou

  2. Institut National de la Santé et de la Recherche Médicale, ESPRI 26, EA4202 UFR Médecine, Université Montpellier 1, Nîmes, France

    Jean-Philippe Lavigne & David O’Callaghan

  3. Department of Biostatistics, Epidemiology, Public Health and Medical Computing, Carémeau University Hospital, Nîmes, France

    Philippe-Jean Bousquet

  4. Department of Obstetrics and Gynecology, Carémeau University Hospital, Nîmes, France

    Pierre Marès & Renaud de Tayrac

Authors
  1. Wassim Badiou
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  2. Jean-Philippe Lavigne
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  3. Philippe-Jean Bousquet
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  4. David O’Callaghan
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  5. Pierre Marès
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  6. Renaud de Tayrac
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Corresponding author

Correspondence to Renaud de Tayrac.

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Badiou, W., Lavigne, JP., Bousquet, PJ. et al. In vitro and in vivo assessment of silver-coated polypropylene mesh to prevent infection in a rat model. Int Urogynecol J 22, 265–272 (2011). https://doi.org/10.1007/s00192-010-1330-y

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  • DOI: https://doi.org/10.1007/s00192-010-1330-y

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