Skip to main content
SpringerLink
Log in

Comparative Assessment of Genotoxic Impacts Induced by Zinc Bulk- and Nano-Particles in Nile tilapia, Oreochromis niloticus

  • Published:
Bulletin of Environmental Contamination and Toxicology Aims and scope Submit manuscript

Abstract

Fish were separately exposed to 1/2 LC50/96 h values of bulk-Zn and nano-Zn for 7, 14, and 28 days. The induction of micronuclei (MN) and other eight nuclear abnormalities in erythrocytes showed marked time and size dependence. The frequencies of all nuclear anomalies were progressively elevated (p < 0.05) with increasing the time of exposure to both bulk-Zn and nano-Zn. Throughout the study periods, fish exposed to nano-Zn showed the maximum elevation in all studied nuclear anomalies. Based on the fragmented DNA values, both Zn forms induced tissue-specific DNA damage as following gills > liver > muscles. Moreover, nano-Zn exposed groups revealed a maximum percentage of DNA damage among all studied groups, especially after 14 days. The percentage of DNA damage was decreased in all tissues on the 28th day, which reflected the presence of an effective repair mechanism. Finally, nano-Zn exhibited more genotoxic effects than that of its bulk counterparts.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Abdel-Khalek AA (2015) Antioxidant responses and nuclear deformations in freshwater fish, Oreochromis niloticus, facing degraded environmental conditions. Bull Environ Contam Toxicol 94:701–708

    Article  CAS  Google Scholar 

  • Abdel-Khalek AA, Kadry M, Hamed A, Marie M-AS (2015) Ecotoxicological impacts of zinc metal in comparison to its nanoparticles in Nile tilapia; Oreochromis niloticus. J Basic Appl Zool 72:113–125

    Article  Google Scholar 

  • Abdel-Khalek AA (2016) Comparative evaluation of genotoxic effects induced by CuO bulk and nano-particles in Nile Tilapia, Oreochromis niloticus. Water Air Soil Pollut 227:35

    Article  Google Scholar 

  • Abdel-Khalek AA, Badran SR, Marie M-AS (2016a) Toxicity evaluation of copper oxide bulk and nanoparticles in Nile tilapia, Oreochromis niloticus, using hematological, bioaccumulation and histological biomarkers. Fish Physiol Biochem 42:1225–1236

    Article  CAS  Google Scholar 

  • Abdel-Khalek AA, Hamed A, Marie M-AS (2016b) The accumulation potency of bulk and nano zinc metal and their impacts on the hematological and histological perturbations of Oreochromis niloticus. Water Air Soil Pollut 227:206

    Article  Google Scholar 

  • Ahamed M, Posgai R, Gorey TJ, Nielsen M, Hussain S, Rowe J (2010) Silver nanoparticles induced heat shock protein 70, oxidative stress and apoptosis in Drosophila melanogaster. Toxicol Appl Pharmacol 242:263–269

    Article  CAS  Google Scholar 

  • American Public Health Association (APHA) (2005) Standard methods for the examination of water and wastewater. American Water Works Association, New York

    Google Scholar 

  • Carriere M, Sauvaigo S, Douki T, Ravanat JL (2017) Impact of nanoparticles on DNA repair processes: current knowledge and working hypotheses. Mutagenesis 32(1):203–213

    Article  CAS  Google Scholar 

  • Chatterjee N, Walker GC (2017) Mechanisms of DNA damage, repair and mutagenesis. Environ Mol Mutagen 58(5):235–263

    Article  CAS  Google Scholar 

  • Doherty AT (2011) The in vitro micronucleus assay. Methods Mol Biol 817:121–141

    Article  Google Scholar 

  • Emmanouil C, Sheehan TMT, Chipman JK (2007) Macromolecule oxidation and DNA repair in mussel (Mytilus edulis L.) gill following exposure to Cd and Cr (VI). Aquat Toxicol 82(1):27–35

    Article  CAS  Google Scholar 

  • Ergene S, Avas TC, Celik A, Koleli N, Kaya F, Karahan A (2007) Monitoring of nuclear abnormalities in peripheral erythrocytes of three fish species from the Goksu Delta (Turkey): genotoxic damage in relation to water pollution. Ecotoxicology 16(4):385–391

    Article  CAS  Google Scholar 

  • Fenech M, Chang WP, Kirsch-Volders M, Holland N, Bonassi S, Zeiger E (2003) HUMN project: detailed description of the scoring criteria for the cytokinesis block micronucleus assay using isolated human lymphocyte cultures. Mutat Res 534(1–2):65–75

    Article  CAS  Google Scholar 

  • Gaiser BK, Biswas A, Rosenkranz P, Jepson MA, Lead JR, Stone V, Tyler CR, Fernandes TF (2011) Effects of silver and cerium dioxide micro- and nano-sized particles on Daphnia magna. J Environ Monitor 13:1227–1235

    Article  CAS  Google Scholar 

  • Gercel-Taylor C, Ackermann MA, Taylor DD (2001) Evaluation of cell proliferation and cell death based assays in chemosensitivity testing. Anticancer Res 21:2761–2768

    CAS  Google Scholar 

  • Hu W, Culloty S, Darmodyb G, Lynch S, Davenport J, Ramirez-Garcia S, Dawsonc KA, Lynch I, Blasco J, Sheehana D (2014) Toxicity of copper oxide nanoparticles in the blue mussel, Mytilus edulis: a redox proteomic investigation. Chemosphere 108:289–299

    Article  CAS  Google Scholar 

  • Isani G, Letizia M, Barucca G, Sekar D, Andreani G, Carpenè E, Falcioni G (2013) Comparative toxicity of CuO nanoparticles and CuSO4 in rainbow trout. Ecotoxicol Environ Saf 97:40–46

    Article  CAS  Google Scholar 

  • Khan MS, Qureshi NA, Jabeen F (2017) Assessment of toxicity in fresh water fish Labeo rohita treated with silver nanoparticles. Appl Nanosci 7:167–179

    Article  CAS  Google Scholar 

  • Kirsch-Volders M, Plas G, Elhajouji A, Lukamowicz M, Gonzalez L, Loock KV (2011) The in vitro MN assay in 2011: origin and fate, biological significance, protocols, high through put methodologies and toxicological relevance. Arch Toxicol 85:873–899

    Article  CAS  Google Scholar 

  • Kumar A, Pandey AK, Singh SS, Shanker R, Dhawan A (2011) Engineered ZnO and TiO2 nanoparticles induce oxidative stress and DNA damage leading to reduced viability of Escherichia coli. Free Radic Biol Med 51:1872–1881

    Article  CAS  Google Scholar 

  • Lebedová J, Hedberg YS, Wallinder IO, Karlsson HL (2018) Size-dependent genotoxicity of silver, gold and platinum nanoparticles studied using the mini-gel comet assay and micronucleus scoring with flow cytometry. Mutagenesis 33:77–85

    Article  Google Scholar 

  • Lindberg HK, Wang X, Järventaus H, Falck GCM, Norppa H, Fenech M (2007) Origin of nuclear buds and micronuclei in normal and folate-deprived human lymphocytes. Mutat Res 617:33–45

    Article  CAS  Google Scholar 

  • Magdolenova Z, Collins A, Kumar A, Dhawan A, Stone V, Dusinska M (2014) Mechanisms of genotoxicity: a review of in vitro and in vivo studies with engineered nanoparticles. Nanotoxicology 8:233–278

    Article  CAS  Google Scholar 

  • Perandones CE, Illera VA, Peckham D, Stunz LL, Ashman RF (1993) Regulation of apoptosis in vitro in mature murine spleen T cells. J Immunol 151:3521–3529

    CAS  Google Scholar 

  • Singh N, Manshian B, Jenkins GJS, Griffiths SM, Williams PM, Maffeis TGG (2009) Nanogenotoxicology: the DNA damaging potential of engineered nanomaterials. Biomaterials 30:3891–3914

    Article  CAS  Google Scholar 

  • Sirelkhatim A, Mahmud S, Seeni A, Kaus NHM, Ann LC, Bakhori SKM, Hasan H, Mohamad D (2015) Review on zinc oxide nanoparticles: antibacterial activity and toxicity mechanism. Nanomicro Lett 7(3):219–242

    CAS  Google Scholar 

  • Vignardi CP, Hasue FM, Sartório PV, Cardoso CM, Machado ASD, Passos MJACR, Santos TCA, Nucci JM, Hewer TLR, Watanabe I-S, Gomes V, Phan NV (2015) Genotoxicity, potential cytotoxicity and cell uptake of titanium dioxide nanoparticles in the marine fish Trachinotus carolinus (Linnaeus, 1766). Aquat Toxicol 158:218–229

    Article  CAS  Google Scholar 

  • Wang Z, Li N, Zhao J, White JC, Qu O, Xing B (2012) CuO nanoparticle interaction with human epithelial cells: cellular uptake, location, export, and genotoxicity. Chem Res Toxicol 25:1512–1521

    Article  CAS  Google Scholar 

  • Zhu B, He W, Hu S, Kong R, Yang L (2019) The fate and oxidative stress of different sized SiO2 nanoparticles in zebrafish (Danio rerio) larvae. Chemosphere 225:705–712

    Article  CAS  Google Scholar 

Download references

Acknowledgement

The authors extend their appreciation to the Deanship of Scientific Research at King Khalid University for funding this work through Research Group Project under grant no. (R.G.P.1–56 − 40), and to the Faculty of Science, Cairo University, Cairo, Egypt for supporting the current work.

Author information

Authors and Affiliations

  1. Zoology Department, Faculty of Science, Cairo University, Cairo, 12613, Egypt

    Amr Adel Abdel-Khalek

  2. Biology Department, College of Science, King Khalid University, Abha, 61421, Saudi Arabia

    Kareem Morsy & Ali Shati

Authors
  1. Amr Adel Abdel-Khalek
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kareem Morsy
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ali Shati
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Amr Adel Abdel-Khalek.

Ethics declarations

Conflicts of interest

The authors declare that they have no conflict of interest.

Ethical approval

This manuscript complies with the ethical rules applicable for this journal. All procedures performed in the present study involving fish were approved (approval no. CUIF4919) and were in accordance with the ethical standards of Faculty of Science, Cairo University, Institutional Animal Care and Use Committee (IACUC) at which the studies were conducted.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Abdel-Khalek, A.A., Morsy, K. & Shati, A. Comparative Assessment of Genotoxic Impacts Induced by Zinc Bulk- and Nano-Particles in Nile tilapia, Oreochromis niloticus. Bull Environ Contam Toxicol 104, 366–372 (2020). https://doi.org/10.1007/s00128-020-02799-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00128-020-02799-9

Keywords

Search

Navigation