Skip to main content
SpringerLink
Log in

Effect of Copper on Growth, Digestive and Antioxidant Enzyme Activities of Juvenile Qihe Crucian Carp, Carassius carassius, During Exposure and Recovery

  • Published:
Bulletin of Environmental Contamination and Toxicology Aims and scope Submit manuscript

Abstract

The toxicity of copper (Cu) on growth and activities of digestive and antioxidant enzymes in the hepatopancreas and intestine of juvenile Qihe crucian carp Carassius carassius was evaluated. The fish were exposed in different Cu solutions for 20 days, and the 0.60 mg/L group was then transferred to clean water to initiate a 20-day recovery period after Cu exposure. Results showed that all enzyme activities decreased significantly at high-concentration (0.30 and 0.60 mg/L) and long-time (20 days) Cu exposures and increased significantly at high-concentration (0.60 mg/L) and short-time Cu exposures (1 day). After the 20-day recovery period, all enzyme activities in the 0.60 mg/L group had recovered to control levels. High-concentration (0.60 mg/L) and long-time (20 days) Cu exposure markedly hindered the growth of fish, whereas the loss of fish growth can not be compensated for by a 20-day recovery period.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Azeez A, Sane AP, Bhatnagar D, Nath P (2007) Enhanced expression of serine proteases during floral senescence in Gladiolus. Phytochemistry 68:1352–1357

    Article  CAS  Google Scholar 

  • Bradford M (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye-binding. Anal Biochem 72:248

    Article  CAS  Google Scholar 

  • Cakmak I, Strbac D, Marschner H (1993) Activities of hydrogen peroxide-scavenging enzymes in germinating wheat seeds. J Exp Bot 44:127–132

    Article  CAS  Google Scholar 

  • Cengiz EI, Unlu E (2006) Sublethal effects of commercial deltamethrin on the structure of the gill, liver and gut tissues of mosquitofish, Gambusia affinis: a microscopic study. Environ Toxicol Phanmacol 21:246–253

    Article  CAS  Google Scholar 

  • Chien YH, Pan CH, Hunter H (2003) The resistance to physical stresses by Penaeus monodon juveniles fed diets supplemented with astaxanthin. Aquaculture 216:177–191

    Article  CAS  Google Scholar 

  • Das PC, Ayyappan S, Jena JK, Das BK (2004) Acute toxicity of ammonia and its sub-lethal effects on selected haematological and enzymatic parameters of mrigal, Cirrhinus mrigala (Hamilton). Aquac Res 35:134–143

    Article  CAS  Google Scholar 

  • Dedourge-Geffard O, Palais F, Biagianti-Risbourg S, Geffard O, Geffard A (2009) Effects of metals on feeding rate and digestive enzymes in Gammarus fossarum: an in situ experiment. Chemosphere 77:1569–1576

    Article  CAS  Google Scholar 

  • GB (1989) Water quality standard for fisheries of the State Standard of the People’s Republic of China: GB11607–89. Ministry of Environmental Protection of the People’s Republic of China, Beijing, China (in Chinese)

  • Hansen JA, Lipton J, Welsh PG, Morris J, Cacela D, Suedkamp MJ (2002) Relationship between exposure duration, tissue residues, growth, and mortality in rainbow trout (Oncorhynchus mykiss) juveniles sub-chronically exposed to copper. Aquat Toxicol 58:175–188

    Article  CAS  Google Scholar 

  • James R, Sampath K, Jothilakshmi S, Vasudhevan I, Thangarathinam R (2008) Effects of copper toxicity on growth, reproduction and metal accumulation in chosen ornamental fishes. Ecohydrol Hydrobiol 8(1):89–97

    Article  CAS  Google Scholar 

  • Jiang HX, Yang HM, Kong XH, Wang SP, Liu DQ, Shi SJ (2012) Response of acid and alkaline phosphatase activities to copper exposure and recovery in freshwater fish Carassius auratus gibelio var. Life Sci J 9(3):233–245

    Google Scholar 

  • Jiang WD, Liu Y, Hua K, Jiang J, Li SH, Feng L, Zhou XQ (2014) Copper exposure induces oxidative injury, disturbs the antioxidant system and changes the Nrf2/ARE (CuZnSOD) signaling in the fish brain: protective effects of myo-inositol. Aquat Toxicol 155:301–313

    Article  CAS  Google Scholar 

  • Kong XH, Jiang HX, Wang SP, Wu XM, Fei W, Li L, Nie GX, Li XJ (2013) Effects of copper exposure on the hatching status and antioxidant defense at different developmental stages of embryos and larvae of goldfish Carassius auratus. Chemosphere 92:1458–1464

    Article  CAS  Google Scholar 

  • Kuz’mina VV, Ushakova NV (2010) The dependence on temperature and pH of the effects of zinc and copper on proteolytic activities of the digestive tract mucosa in piscivorous fish and their potential preys. Fish Physiol Biochem 36:787–795

    Article  Google Scholar 

  • Li LJ, Liu XM, Guo YP, Ma EB (2005) Activity of the enzymes of the antioxidative system in cadmium-treated Oxya chinensis (Orthoptera Acridoidae). Environ Toxicol Pharmacol 20:412–416

    Article  CAS  Google Scholar 

  • Li JS, Li JL, Wu TT (2007) The effects of copper, iron and zinc on digestive enzyme activity in the hybrid tilapia Oreochromis niloticus (L.) × Oreochromis aureus (Steindachner). J Fish Biol 71:1788–1798

    Article  CAS  Google Scholar 

  • Li N, Zhao YL, Yang J (2008) Effects of water-borne copper on digestive and metabolic enzymes of the giant freshwater prawn Macrobrachium rosenbergii. Arch Environ Contam Toxicol 55:86–93

    Article  CAS  Google Scholar 

  • Liu C, Fu JX, Xue F, Zhao YH, Zhu ZF (2007) Experimental studies on the influencing factors of algae killing by copper sulfate. Contemp Chem Ind 5:488–491 (in Chinese)

    Google Scholar 

  • Liu XJ, Luo Z, Xiong BX, Liu X, Zhao YH, Hu GF, Lv GJ (2010) Effect of waterborne copper exposure on growth, hepatic enzymatic activities and histology in Synechogobius hasta. Ecotoxicol Environ Saf 73:1286–1291

    Article  CAS  Google Scholar 

  • Mastin BJ, Rodgers JH (2000) Toxicity and bioavailability of copper herbicides (Clearigate, Cutrine-Plus, and copper sulfate) to freshwater animals. Arch Environ Contam Toxicol 39:445–451

    Article  CAS  Google Scholar 

  • Muhlia-Almazán A, García-Carreño FL (2002) Influence of molting and starvation on the synthesis of proteolytic enzymes in the midgut gland of the white shrimp Penaeus vannamei. Comp Biochem Physiol 133:383–394

    Article  Google Scholar 

  • Orbea A, Ortiz-Zarragoitia M, Solé M, Porte C, Cajaraville MP (2002) Antioxidant enzymes and peroxisome proliferation in relation to contaminant body burdens of PAHs and PCBs in bivalve molluscs, crabs and fish from the Urdaibai and Plentzia estuaries (Bay of Biscay). Aquat Toxicol 58:75–98

    Article  CAS  Google Scholar 

  • Oruc EO, Sevgiler Y, Uner N (2004) Tissue-specific oxidative stress responses in fish exposed to 2,4-D and azinphosmethyl. Comp Biochem Physiol 137C:43–51

    Google Scholar 

  • Rick W, Stegbauer HP (1984) Alfa-amylase. In: Bergmeyer HU, Grab M (eds) Methods of enzymatic analysis. Enzymes, vol 5. Academic Press, New York, pp 885–889

    Google Scholar 

  • Ross SW, Dalton DA, Kranmer S, Christensen BL (2001) Physiological (antioxidant) responses of estuarine fishes to variability in dissolved oxygen. Comp Biochem Physiol 130C:289–303

    CAS  Google Scholar 

  • Tang QQ, Feng L, Jiang WD, Liu Y, Jiang J, Li SH, Kuang SY, Tang L, Zhou XQ (2013) Effects of dietary copper on growth, digestive, and brush border enzyme activities and antioxidant defense of hepatopancreas and intestine for young grass carp (Ctenopharyngodon idella). Biol Trace Elem Res 155:370–380

    Article  CAS  Google Scholar 

  • Tsuzuki MY, Sugai JK, Maciel JC, Francisco CJ, Cerqueira VR (2007) Survival, growth and digestive enzyme activity of juveniles of the fat snook (Centropomus parallelus) reared at different salinities. Aquaculture 271:319–325

    Article  CAS  Google Scholar 

  • Von Borell E (2000) Stress and coping in farm animals. Arch Tierz (Sonderheft) 43:144–152

    Google Scholar 

  • Vutukuru SS, Chintada S, Madhavi KR, Rao JV, Anjaneyulu Y (2006) Acute effects of copper on superoxide dismutase, catalase and lipid peroxidation in the freshwater teleost fish, Esomus danricus. Fish Physiol Biochem 32:221–229

    Article  CAS  Google Scholar 

  • Wang L, Yan B, Liu N, Li Y, Wang Q (2008) Effects of cadmium on glutathione synthesis in hepatopancreas of freshwater crab, Sinopotamon yangtsekiense. Chemosphere 74:51–56

    Article  CAS  Google Scholar 

  • Wang T, Long XH, Cheng YZ, Liu ZP, Yan SH (2015) A comparison effect of copper nanoparticles versus copper sulphate on juvenile Epinephelus coioides: growth parameters, digestive enzymes, body composition, and histology as biomarkers. Int J Genomics 14:1–10

    Google Scholar 

  • Ye JS, Chen XJ, Zhu YY (2013) Influence of pH on survival, growth and activities of digestive enzymes of Odontobutis obscures. Adv J Food Sci Technol 5:1234–1237

    Google Scholar 

  • Zhang JJ, Yang XB (2012) Fishery water quality evaluation of Qihe crucian carp national aquatic germplasm resources conservation area in Henan province. Henan Fish 1:40–42 (in Chinese)

    CAS  Google Scholar 

  • Zhang JF, Shen H, Xu TL, Wang XR, Li WM, Gu YF (2003) Effects of long-term exposure of low-level diesel oil on the antioxidant defense system of fish. Bull Environ Contam Toxicol 71:234–239

    Article  CAS  Google Scholar 

  • Zhang JF, Shen H, Wang XR, Wu JC, Xue YQ (2004) Effects of chronic exposure of 2,4-dichlorophenol on the antioxidant system in liver of fresh water fish Carassius auratus. Chemosphere 55:167–174

    Article  CAS  Google Scholar 

  • Zhao HL, Xiao TY (2007) The resources status and protection countermeasures of Qihe crucian carp. Henan Fish 3:5–6 (in Chinese)

    Google Scholar 

Download references

Acknowledgments

This work was sponsored by the Program for Science and Technology Innovation Talents in Universities of Henan Province (Grant No. 2011HASTIT012) and the Key Projects of Science and Technology Research of the Department of Education in Henan Province (Grant No. 13A240509). This study was also partly supported by the Key Subjects of Fisheries in Henan Province, China.

Author information

Authors and Affiliations

  1. College of Fisheries, Henan Normal University, No. 46 Jianshe East Road, Xinxiang City, 453007, People’s Republic of China

    Hongxia Jiang, Xianghui Kong, Shuping Wang & Huiyun Guo

Authors
  1. Hongxia Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xianghui Kong
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shuping Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Huiyun Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xianghui Kong.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Jiang, H., Kong, X., Wang, S. et al. Effect of Copper on Growth, Digestive and Antioxidant Enzyme Activities of Juvenile Qihe Crucian Carp, Carassius carassius, During Exposure and Recovery. Bull Environ Contam Toxicol 96, 333–340 (2016). https://doi.org/10.1007/s00128-016-1738-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00128-016-1738-2

Keywords

Search

Navigation